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10.1016/s0022-1759(01)00455-0 http://scihub22266oqcxt.onion/10.1016/s0022-1759(01)00455-0 11687249!ä!11687249 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol+Methods 2001 ; 257 (1-2): 155-61 Nephropedia Template TP {{tp|p=11687249|t=2001. An improved protocol for measuring cytotoxic T cell activity in anatomic compartments with low cell numbers |pdf=|usr=}}{{11687249}} gab.com Text An improved protocol for measuring cytotoxic T cell activity in anatomic compartments with low cell numbers JO: J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=11687249 #FOAvip The study of target cell lysis and cytokine production are valuable tools to characterize antigen-specific T and NK cell function during virus infections. After localized infections in compartments such as the lung or the brain, however, cell numbers isolated from these organs are too low to perform standard assays with individual mice. Here, we report a few simple modifications of the classical 51Cr release assay allowing reduction of the number of required effector cells by a factor of 10 without loosing sensitivity or specificity. Using not more than 4x10(5) effector cells, we were able to study ex vivo virus-specific CTL or NK activity from the lungs of individual mice after infection with respiratory syncytial virus (RSV) and from the brains of mice infected with Borna disease virus (BDV). Flow cytometric analysis of interferon-gamma production by virus-specific T cells including appropriate controls was achieved with as few as 10(5) effector cells. Twit Text FOAVip An improved protocol for measuring cytotoxic T cell activity in anatomic compartments with low cell numbers ????J Immunol Methods http://www.kidney.de/pget.php?&tw=1&pmid=11687249 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=11687249 #FOAvip English Wikipedia <ref>{{Cite pmid|11687249}}</ref> An improved protocol for measuring cytotoxic T cell activity in anatomic compartments with low cell numbers #MMPMID11687249 Ostler T; Schamel K; Hussell T; Openshaw P; Hausmann J; Ehl S J Immunol Methods 2001[Nov]; 257 (1-2): 155-61 PMID11687249show ga The study of target cell lysis and cytokine production are valuable tools to characterize antigen-specific T and NK cell function during virus infections. After localized infections in compartments such as the lung or the brain, however, cell numbers isolated from these organs are too low to perform standard assays with individual mice. Here, we report a few simple modifications of the classical 51Cr release assay allowing reduction of the number of required effector cells by a factor of 10 without loosing sensitivity or specificity. Using not more than 4x10(5) effector cells, we were able to study ex vivo virus-specific CTL or NK activity from the lungs of individual mice after infection with respiratory syncytial virus (RSV) and from the brains of mice infected with Borna disease virus (BDV). Flow cytometric analysis of interferon-gamma production by virus-specific T cells including appropriate controls was achieved with as few as 10(5) effector cells. |Animals[MESH] |Borna Disease/immunology/pathology[MESH] |Brain/cytology/immunology[MESH] |Bronchoalveolar Lavage Fluid/cytology[MESH] |Cell Line[MESH] |Cytotoxicity Tests, Immunologic/*methods[MESH] |Flow Cytometry[MESH] |In Vitro Techniques[MESH] |Killer Cells, Natural/immunology[MESH] |Lung/cytology/immunology[MESH] |Lymphocyte Count[MESH] |Lymphocytic Choriomeningitis/immunology/pathology[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Mice, Inbred C57BL[MESH] |Organ Specificity[MESH] |Respiratory Syncytial Virus Infections/immunology/pathology[MESH]An improved protocol for measuring cytotoxic T cell activity in anatom(epmc).pdf DeepDyve Pubget Overpricing