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10.1099/0022-1317-82-3-641

http://scihub22266oqcxt.onion/10.1099/0022-1317-82-3-641
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11172106!ä!11172106

suck abstract from ncbi


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pmid11172106      J+Gen+Virol 2001 ; 82 (Pt 3): 641-646
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  • Identification and characterization of a new intron in Borna disease virus #MMPMID11172106
  • Cubitt B; Ly C; de la Torre JC
  • J Gen Virol 2001[Mar]; 82 (Pt 3): 641-646 PMID11172106show ga
  • Borna disease virus (BDV) has a non-segmented, negative-strand (NNS) RNA genome. In contrast to all other known NNS RNA animal viruses, BDV replication and transcription occur in the nucleus of infected cells. Moreover, BDV uses RNA splicing for the regulation of its genome expression. Two introns (I and II), both present in two viral primary transcripts of 2.5 and 7.2 kb, have been reported in BDV. Here, evidence is provided of a new BDV intron, intron III, generated by alternative 3' splice-site choice. Intron III-spliced mRNAs were detected at early times post-infection and found to be present in cells from different types and species. Intron III-spliced mRNAs have coding capability for two new viral proteins with predicted molecular masses of 8.4 and 165 (p165) kDa. p165 is a deleted form of the BDV L polymerase, containing three RGD motifs and a signal peptide signal that could target it into the secretory pathway. These findings underscore the proteomic complexity exhibited by BDV.
  • |*Introns[MESH]
  • |Alternative Splicing[MESH]
  • |Animals[MESH]
  • |Borna disease virus/*genetics[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Humans[MESH]
  • |RNA, Viral[MESH]
  • |Rats[MESH]
  • |Tumor Cells, Cultured[MESH]


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