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10.1006/viro.2000.0673

http://scihub22266oqcxt.onion/10.1006/viro.2000.0673
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11118359!ä!11118359

suck abstract from ncbi


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pmid11118359      Virology 2000 ; 278 (2): 361-7
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  • Different patterns of restriction to B19 parvovirus replication in human blast cell lines #MMPMID11118359
  • Gallinella G; Manaresi E; Zuffi E; Venturoli S; Bonsi L; Bagnara GP; Musiani M; Zerbini M
  • Virology 2000[Dec]; 278 (2): 361-7 PMID11118359show ga
  • B19 parvovirus can replicate in erythroid progenitor cells and in a small number of human blast cell lines. To better understand and analyze the B19 virus replicative cycle, we performed and compared the infection of bone marrow cells and of different blast cell lines with erythroblastoid and megakaryoblastoid phenotypic characteristics (UT-7, TF-1, M-07, and B1647). Following in vitro infection, B19-specific nucleic acids were characterized with regard to the genome-replicative intermediates, the transcription pattern, and the localization of virus-specific nucleic acids inside infected cells. While all cell lines tested proved to be susceptible to B19 virus infection, two different patterns of restriction to replication of B19 virus were observed. In the first restriction pattern, observed in UT-7 cells, the single-stranded viral DNA was converted to double-stranded replicative intermediates, identical to those found in bone marrow cells, and a full set of viral transcripts were observed. However, replication and transcription were restricted to a small subset of cells, and production of capsid proteins was not detected. In the second restriction pattern, observed in TF-1, M-07, and B1647 cells, the single-stranded viral DNA was not converted to double-stranded replicative intermediates.
  • |*Virus Replication[MESH]
  • |Bone Marrow Cells/cytology/*virology[MESH]
  • |Cell Line[MESH]
  • |Cells, Cultured[MESH]
  • |DNA, Viral/genetics[MESH]
  • |Erythroid Precursor Cells/cytology/*virology[MESH]
  • |Genome, Viral[MESH]
  • |Humans[MESH]
  • |In Situ Hybridization[MESH]
  • |Megakaryocytes/cytology/*virology[MESH]
  • |Parvovirus B19, Human/genetics/*physiology[MESH]
  • |RNA, Viral/genetics[MESH]


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