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10.1152/ajpcell.2000.279.6.C1955 http://scihub22266oqcxt.onion/10.1152/ajpcell.2000.279.6.C1955 11078711!ä!11078711 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol+Cell+Physiol 2000 ; 279 (6): C1955-62 Nephropedia Template TP {{tp|p=11078711|t=2000. Sodium gradient-dependent transport of magnesium in rat ventricular myocytes |pdf=|usr=}}{{11078711}} gab.com Text Sodium gradient-dependent transport of magnesium in rat ventricular myocytes JO: Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=11078711 #FOAvip Cytoplasmic concentration of Mg(2+) ([Mg(2+)](i)) was measured with a fluorescent indicator furaptra in ventricular myocytes enzymatically dissociated from rat hearts (25 degrees C). To study Mg(2+) transport across the cell membrane, cells were treated with ionomycin in Ca(2+)-free (0.1 mM EGTA) and high-Mg(2+) (10 mM) conditions to facilitate passive Mg(2+) influx. Rate of rise of [Mg(2+)](i) due to the net Mg(2+) influx was significantly smaller in the presence of 130 mM extracellular Na(+) than in its absence. We also tested the extracellular Na(+) dependence of the net Mg(2+) efflux from cells loaded with Mg(2+). After [Mg(2+)](i) was raised by ionomycin and high Mg(2+) to the level 0.5-0.6 mM above the basal value ( approximately 0.7 mM), washout of ionomycin and lowering extracellular [Mg(2+)] to 1.2 mM caused rapid decline of [Mg(2+)](i) in the presence of 140 mM Na(+). This net efflux of Mg(2+) was completely inhibited by withdrawal of extracellular Na(+) and was largely attenuated by imipramine, a known inhibitor of Na(+)/Mg(2+) exchange, with 50% inhibition at 79 microM. The relation between the rate of net Mg(2+) efflux and extracellular Na(+) concentration ([Na(+)](o)) had a Hill coefficient of 2 and [Na(+)](o) at half-maximal rate of 82 mM. These results demonstrate the presence of Na(+) gradient-dependent Mg(2+) transport, which is consistent with Na(+)/Mg(2+) exchange, in cardiac myocytes. Twit Text FOAVip Sodium gradient-dependent transport of magnesium in rat ventricular myocytes ????Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=11078711 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=11078711 #FOAvip English Wikipedia <ref>{{Cite pmid|11078711}}</ref> Sodium gradient-dependent transport of magnesium in rat ventricular myocytes #MMPMID11078711 Tashiro M; Konishi M Am J Physiol Cell Physiol 2000[Dec]; 279 (6): C1955-62 PMID11078711show ga Cytoplasmic concentration of Mg(2+) ([Mg(2+)](i)) was measured with a fluorescent indicator furaptra in ventricular myocytes enzymatically dissociated from rat hearts (25 degrees C). To study Mg(2+) transport across the cell membrane, cells were treated with ionomycin in Ca(2+)-free (0.1 mM EGTA) and high-Mg(2+) (10 mM) conditions to facilitate passive Mg(2+) influx. Rate of rise of [Mg(2+)](i) due to the net Mg(2+) influx was significantly smaller in the presence of 130 mM extracellular Na(+) than in its absence. We also tested the extracellular Na(+) dependence of the net Mg(2+) efflux from cells loaded with Mg(2+). After [Mg(2+)](i) was raised by ionomycin and high Mg(2+) to the level 0.5-0.6 mM above the basal value ( approximately 0.7 mM), washout of ionomycin and lowering extracellular [Mg(2+)] to 1.2 mM caused rapid decline of [Mg(2+)](i) in the presence of 140 mM Na(+). This net efflux of Mg(2+) was completely inhibited by withdrawal of extracellular Na(+) and was largely attenuated by imipramine, a known inhibitor of Na(+)/Mg(2+) exchange, with 50% inhibition at 79 microM. The relation between the rate of net Mg(2+) efflux and extracellular Na(+) concentration ([Na(+)](o)) had a Hill coefficient of 2 and [Na(+)](o) at half-maximal rate of 82 mM. These results demonstrate the presence of Na(+) gradient-dependent Mg(2+) transport, which is consistent with Na(+)/Mg(2+) exchange, in cardiac myocytes. |Adrenergic Uptake Inhibitors/pharmacology[MESH] |Amiloride/pharmacology[MESH] |Animals[MESH] |Biological Transport/drug effects/physiology[MESH] |Chelating Agents/pharmacology[MESH] |Diuretics/pharmacology[MESH] |Egtazic Acid/pharmacology[MESH] |Extracellular Space[MESH] |Imipramine/pharmacology[MESH] |In Vitro Techniques[MESH] |Ionomycin/pharmacology[MESH] |Ionophores/pharmacokinetics[MESH] |Lithium/pharmacology[MESH] |Magnesium/*pharmacokinetics[MESH] |Male[MESH] |Muscle Fibers, Skeletal/*metabolism[MESH] |Myocardium/cytology/*metabolism[MESH] |Potassium/pharmacology[MESH] |Rats[MESH] |Rats, Wistar[MESH]Sodium gradient-dependent transport of magnesium in rat ventricular my(epmc).pdf DeepDyve Pubget Overpricing