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10.1006/viro.2000.0460 http://scihub22266oqcxt.onion/10.1006/viro.2000.0460 10964772!ä!10964772 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 2000 ; 274 (2): 284-91 Nephropedia Template TP {{tp|p=10964772|t=2000. Identification of a novel RNA splicing pattern as a basis of restricted cell tropism of erythrovirus B19 |pdf=|usr=}}{{10964772}} gab.com Text Identification of a novel RNA splicing pattern as a basis of restricted cell tropism of erythrovirus B19 JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=10964772 #FOAvip Prior studies on the transcription of erythrovirus B19 have identified a short leader sequence associated with all spliced viral transcripts. While some variability has been observed in the acceptor for this first intron, studies to date in both permissive and nonpermissive cell types have reported a unique splice donor site. In the semipermissive MB-02 cell line, we have found that splicing of this first intron proceeds almost exclusively via a cryptic CT donor downstream of the previously reported GT donor at nucleotide 406. The resulting messages for the viral structural proteins and 11-kDa protein are thereby made bicistronic, with the first expressible polypeptide being a 34 amino acid fusion of the NS-1 and 7.5-kDa proteins. The presence of an upstream open-reading frame on these messages is likely to block effective translation of the downstream structural protein products. We propose this as a significant mechanism in determining B19's tropism on the basis of host cell splicing machinery, and present evidence in support of this model. Additionally, this is the first report of usage of a noncanonical splice donor in B19, and to our knowledge the first report of a CT-AG splice in any system. Twit Text FOAVip Identification of a novel RNA splicing pattern as a basis of restricted cell tropism of erythrovirus B19 ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=10964772 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=10964772 #FOAvip English Wikipedia <ref>{{Cite pmid|10964772}}</ref> Identification of a novel RNA splicing pattern as a basis of restricted cell tropism of erythrovirus B19 #MMPMID10964772 Brunstein J; Soderlund-Venermo M; Hedman K Virology 2000[Sep]; 274 (2): 284-91 PMID10964772show ga Prior studies on the transcription of erythrovirus B19 have identified a short leader sequence associated with all spliced viral transcripts. While some variability has been observed in the acceptor for this first intron, studies to date in both permissive and nonpermissive cell types have reported a unique splice donor site. In the semipermissive MB-02 cell line, we have found that splicing of this first intron proceeds almost exclusively via a cryptic CT donor downstream of the previously reported GT donor at nucleotide 406. The resulting messages for the viral structural proteins and 11-kDa protein are thereby made bicistronic, with the first expressible polypeptide being a 34 amino acid fusion of the NS-1 and 7.5-kDa proteins. The presence of an upstream open-reading frame on these messages is likely to block effective translation of the downstream structural protein products. We propose this as a significant mechanism in determining B19's tropism on the basis of host cell splicing machinery, and present evidence in support of this model. Additionally, this is the first report of usage of a noncanonical splice donor in B19, and to our knowledge the first report of a CT-AG splice in any system. |*Virus Replication[MESH] |Alternative Splicing/*genetics[MESH] |Amino Acid Sequence[MESH] |Base Sequence[MESH] |Cell Line[MESH] |Cloning, Molecular[MESH] |Humans[MESH] |Introns/genetics[MESH] |Molecular Sequence Data[MESH] |Open Reading Frames/genetics[MESH] |Organ Specificity[MESH] |Parvovirus B19, Human/*genetics/*physiology[MESH] |Polymerase Chain Reaction[MESH] |Protein Biosynthesis[MESH] |RNA, Messenger/analysis/genetics[MESH] |RNA, Spliced Leader/analysis/*genetics[MESH] |RNA, Viral/analysis/*genetics[MESH] |Retinoblastoma/genetics[MESH] |Ribonuclease, Pancreatic/metabolism[MESH] |Ribosomes/metabolism[MESH] |Templates, Genetic[MESH] |Transcription, Genetic/genetics[MESH]Identification of a novel RNA splicing pattern as a basis of restricte(epmc).pdf DeepDyve Pubget Overpricing