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10.1073/pnas.97.14.8157 http://scihub22266oqcxt.onion/10.1073/pnas.97.14.8157 10884438!16686!10884438     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2000 ; 97 (14): 8157-62 Nephropedia Template TP {{tp|p=10884438|t=2000. Deranged transcriptional regulation of cell-volume-sensitive kinase hSGK in diabetic nephropathy |pdf=|usr=}}{{10884438}} gab.com Text Deranged transcriptional regulation of cell-volume-sensitive kinase hSGK in diabetic nephropathy JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=10884438 #FOAvip Transforming growth factor beta (TGF-beta) has been shown to participate in the pathophysiology of diabetic complications. As shown most recently, TGF-beta stimulates the expression of a distinct serine/threonine kinase (hSGK) which had previously been cloned as an early gene transcriptionally regulated by cell volume alterations. The present study was performed to elucidate transcription and function of hSGK in diabetic nephropathy. As shown by Northern blotting, an increase of extracellular glucose concentration increased hSGK mRNA levels in cultured cells, an effect qualitatively mimicked by osmotic cell shrinkage or treatment with TGF-beta (2 microgram/liter), phorbol 12,13-didecanoate (1 microM), or the Ca(2+) ionophore ionomycin (1 microM) and blunted by high concentrations of nifedipine (10 and 100 microM). In situ hybridization revealed that hSGK transcription was markedly enhanced in diabetic nephropathy, with particularly high expression in mesangial cells, interstitial cells, and cells in thick ascending limbs of Henle's loop and distal tubules. According to voltage clamp and tracer flux studies in Xenopus oocytes expressing the renal epithelial Na(+) channel ENaC or the mouse thick ascending limb Na(+),K(+),2Cl(-) cotransporter BSC-1, coexpression with hSGK stimulated ENaC and BSC-1 11-fold and 6-fold, respectively, effects reversed by kinase inhibitors staurosporine (1 microM) and chelerythrine (1 microM) and not elicited by inactive hSGK. In conclusion, excessive extracellular glucose concentrations enhance hSGK transcription, which in turn stimulates renal tubular Na(+) transport. These observations disclose an additional element in the pathophysiology of diabetic nephropathy. Twit Text FOAVip Deranged transcriptional regulation of cell-volume-sensitive kinase hSGK in diabetic nephropathy ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=10884438 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=10884438 #FOAvip English Wikipedia <ref>{{Cite pmid|10884438}}</ref> Deranged transcriptional regulation of cell-volume-sensitive kinase hSGK in diabetic nephropathy #MMPMID10884438 Lang F; Klingel K; Wagner CA; Stegen C; Warntges S; Friedrich B; Lanzendorfer M; Melzig J; Moschen I; Steuer S; Waldegger S; Sauter M; Paulmichl M; Gerke V; Risler T; Gamba G; Capasso G; Kandolf R; Hebert SC; Massry SG; Broer S Proc Natl Acad Sci U S A 2000[Jul]; 97 (14): 8157-62 PMID10884438show ga Transforming growth factor beta (TGF-beta) has been shown to participate in the pathophysiology of diabetic complications. As shown most recently, TGF-beta stimulates the expression of a distinct serine/threonine kinase (hSGK) which had previously been cloned as an early gene transcriptionally regulated by cell volume alterations. The present study was performed to elucidate transcription and function of hSGK in diabetic nephropathy. As shown by Northern blotting, an increase of extracellular glucose concentration increased hSGK mRNA levels in cultured cells, an effect qualitatively mimicked by osmotic cell shrinkage or treatment with TGF-beta (2 microgram/liter), phorbol 12,13-didecanoate (1 microM), or the Ca(2+) ionophore ionomycin (1 microM) and blunted by high concentrations of nifedipine (10 and 100 microM). In situ hybridization revealed that hSGK transcription was markedly enhanced in diabetic nephropathy, with particularly high expression in mesangial cells, interstitial cells, and cells in thick ascending limbs of Henle's loop and distal tubules. According to voltage clamp and tracer flux studies in Xenopus oocytes expressing the renal epithelial Na(+) channel ENaC or the mouse thick ascending limb Na(+),K(+),2Cl(-) cotransporter BSC-1, coexpression with hSGK stimulated ENaC and BSC-1 11-fold and 6-fold, respectively, effects reversed by kinase inhibitors staurosporine (1 microM) and chelerythrine (1 microM) and not elicited by inactive hSGK. In conclusion, excessive extracellular glucose concentrations enhance hSGK transcription, which in turn stimulates renal tubular Na(+) transport. These observations disclose an additional element in the pathophysiology of diabetic nephropathy. |3T3 Cells[MESH] |Animals[MESH] |Carrier Proteins/biosynthesis/genetics[MESH] |Cell Size[MESH] |Diabetic Nephropathies/*genetics[MESH] |Epithelial Sodium Channels[MESH] |Gene Expression Regulation[MESH] |Humans[MESH] |In Situ Hybridization[MESH] |Kidney/pathology[MESH] |Mice[MESH] |Patch-Clamp Techniques[MESH] |Protein Serine-Threonine Kinases/biosynthesis/*genetics[MESH] |Rats[MESH] |Recombinant Proteins/biosynthesis[MESH] |Sodium Channels/biosynthesis/genetics[MESH] |Sodium-Potassium-Chloride Symporters[MESH] |Sodium/metabolism[MESH] |Tissue Distribution[MESH]Deranged transcriptional regulation of cell-volume-sensitive kinase hS(epmc).pdf DeepDyve Pubget Overpricing