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10.1006/mpat.1999.0311 http://scihub22266oqcxt.onion/10.1006/mpat.1999.0311 10588909!ä!10588909 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=10588909&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microb+Pathog 1999 ; 27 (6): 369-76 Nephropedia Template TP {{tp|p=10588909|t=1999. The Legionella pneumophila prp locus; required during infection of macrophages and amoebae |pdf=|usr=}}{{10588909}} gab.com Text The Legionella pneumophila prp locus; required during infection of macrophages and amoebae JO: Microb Pathog http://www.kidney.de/pget.php?&tw=1&pmid=10588909 #FOAvip Transposon mutagenesis was performed using mTn 10phoA to identify Legionella pneumophila genes that are expressed under certain in vitro conditions, and are required for intracellular replication. Of the 1653 PhoA fusions examined, 19 PhoA(+)fusion mutants were isolated and screened for differential expression of fusion proteins after growth at 30 or 37 degrees C, in the presence of low iron, or increased magnesium concentrations. The mutants were examined for their cytopathogenicity and intracellular replication within U937 macrophage-like cells and the protozoan Hartmannella vermiformis. One of the mutants generated, BS10, was defective in its multiplication within U937 macrophage-like cells and H. vermiformis. The defect in BS10 was complemented with a cosmid clone containing the wild type locus. The open reading frame interrupted by the insertion was homologous to prpD of Salmonella typhimurium and mmgE of Bacillus subtilis. Twit Text FOAVip The Legionella pneumophila prp locus; required during infection of macrophages and amoebae ????Microb Pathog http://www.kidney.de/pget.php?&tw=1&pmid=10588909 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=10588909 #FOAvip English Wikipedia <ref>{{Cite pmid|10588909}}</ref> The Legionella pneumophila prp locus; required during infection of macrophages and amoebae #MMPMID10588909 Stone BJ; Brier A; Kwaik YA Microb Pathog 1999[Dec]; 27 (6): 369-76 PMID10588909show ga Transposon mutagenesis was performed using mTn 10phoA to identify Legionella pneumophila genes that are expressed under certain in vitro conditions, and are required for intracellular replication. Of the 1653 PhoA fusions examined, 19 PhoA(+)fusion mutants were isolated and screened for differential expression of fusion proteins after growth at 30 or 37 degrees C, in the presence of low iron, or increased magnesium concentrations. The mutants were examined for their cytopathogenicity and intracellular replication within U937 macrophage-like cells and the protozoan Hartmannella vermiformis. One of the mutants generated, BS10, was defective in its multiplication within U937 macrophage-like cells and H. vermiformis. The defect in BS10 was complemented with a cosmid clone containing the wild type locus. The open reading frame interrupted by the insertion was homologous to prpD of Salmonella typhimurium and mmgE of Bacillus subtilis. |Alkaline Phosphatase[MESH] |Animals[MESH] |Cell Line[MESH] |Colony Count, Microbial[MESH] |Culture Media[MESH] |Cyclin-Dependent Kinases/genetics/metabolism[MESH] |DNA Transposable Elements[MESH] |Genetic Complementation Test[MESH] |Hartmannella/*microbiology[MESH] |Humans[MESH] |Iron/metabolism[MESH] |Legionella pneumophila/*genetics/growth & development/*pathogenicity[MESH] |Macrophages/*microbiology[MESH] |Magnesium/metabolism[MESH] |Mutagenesis, Insertional[MESH] |Open Reading Frames[MESH] |Temperature[MESH]The Legionella pneumophila prp locus; required during infection of mac(epmc).pdf DeepDyve Pubget Overpricing