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10.1292/jvms.61.831 http://scihub22266oqcxt.onion/10.1292/jvms.61.831 10458109!ä!10458109 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Vet+Med+Sci 1999 ; 61 (7): 831-4 Nephropedia Template TP {{tp|p=10458109|t=1999. Enhancement of Borna disease virus transcription in persistently infected cells by serum starvation |pdf=|usr=}}{{10458109}} gab.com Text Enhancement of Borna disease virus transcription in persistently infected cells by serum starvation JO: J Vet Med Sci http://www.kidney.de/pget.php?&tw=1&pmid=10458109 #FOAvip Transcription of Borna disease virus (BDV) in persistently infected MDCK (MDCK/BDV) cells increased in the fetal bovine serum free media as detected by Northern blot analysis. Especially, the amount of 1.9-kb RNA without cap formation at the 5' end and polyadenylation at the 3' end, increased as compared to other mRNA molecules of BDV. Growth arrest of MDCK/BDV cells observed in the condition of serum starvation might be important for increasing viral transcription. Since N-cadherin is the responsible factor for cell-to-cell contact, MDCK/BDV cells were cultured in calcium free medium which inhibits the interaction of N-cadherin. However, inhibition of cell-to-cell contact by N-cadherin is not effective on up regulation of viral transcription. Our finding in this study indicates that enhancement of BDV transcription by serum starvation is a useful technique for further investigation in understanding of mechanisms of BDV transcription. Twit Text FOAVip Enhancement of Borna disease virus transcription in persistently infected cells by serum starvation ????J Vet Med Sci http://www.kidney.de/pget.php?&tw=1&pmid=10458109 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=10458109 #FOAvip English Wikipedia <ref>{{Cite pmid|10458109}}</ref> Enhancement of Borna disease virus transcription in persistently infected cells by serum starvation #MMPMID10458109 Mizutani T; Inagaki H; Hayasaka D; Kariwa H; Takashima I J Vet Med Sci 1999[Jul]; 61 (7): 831-4 PMID10458109show ga Transcription of Borna disease virus (BDV) in persistently infected MDCK (MDCK/BDV) cells increased in the fetal bovine serum free media as detected by Northern blot analysis. Especially, the amount of 1.9-kb RNA without cap formation at the 5' end and polyadenylation at the 3' end, increased as compared to other mRNA molecules of BDV. Growth arrest of MDCK/BDV cells observed in the condition of serum starvation might be important for increasing viral transcription. Since N-cadherin is the responsible factor for cell-to-cell contact, MDCK/BDV cells were cultured in calcium free medium which inhibits the interaction of N-cadherin. However, inhibition of cell-to-cell contact by N-cadherin is not effective on up regulation of viral transcription. Our finding in this study indicates that enhancement of BDV transcription by serum starvation is a useful technique for further investigation in understanding of mechanisms of BDV transcription. |*Culture Media, Serum-Free[MESH] |*Gene Expression Regulation, Viral[MESH] |*Transcription, Genetic[MESH] |Animals[MESH] |Borna disease virus/*genetics[MESH] |Cadherins/pharmacology[MESH] |Calcium/physiology[MESH] |Cattle[MESH] |Cell Communication[MESH] |Cell Division[MESH] |Cell Line[MESH] |Genome, Viral[MESH]Enhancement of Borna disease virus transcription in persistently infec(epmc).pdf DeepDyve Pubget Overpricing