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10.1074/jbc.274.15.10654 http://scihub22266oqcxt.onion/10.1074/jbc.274.15.10654 10187863!ä!10187863 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 1999 ; 274 (15): 10654-60 Nephropedia Template TP {{tp|p=10187863|t=1999. Ceramide generation in nitric oxide-induced apoptosis Activation of magnesium-dependent neutral sphingomyelinase via caspase-3 |pdf=|usr=}}{{10187863}} gab.com Text Ceramide generation in nitric oxide-induced apoptosis Activation of magnesium-dependent neutral sphingomyelinase via caspase-3 JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=10187863 #FOAvip Sodium nitroprusside (SNP), a NO donor, has been recognized as an inducer of apoptosis in various cell lines. Here, we demonstrated the intracellular formation of ceramide, a lipid signal mediator, in SNP-induced apoptosis in human leukemia HL-60 cells and investigated the mechanisms of ceramide generation. The levels of intracellular ceramide increased to, at most, 160% of the control level in a time- and dose-dependent manner when the cells were treated with 1 mM SNP. SNP also decreased the sphingomyelin level to approximately 70% of the control level and increased magnesium-dependent neutral sphingomyelinase (N-SMase) activity to 160% of the control activity 2 h after treatment. Neither acid SMase nor magnesium-independent N-SMase was affected by SNP. Caspases are thought to be key enzymes in apoptotic cell death. Acetyl-Asp-Glu-Val-Asp-aldehyde, a synthetic tetrapeptide inhibitor of caspases, inhibited magnesiumdependent N-SMase, ceramide generation, and apoptosis. Moreover, recombinant purified caspase-3 increased magnesium-dependent N-SMase in a cell-free system. These results suggest that the findings that SNP increased ceramide generation and magnesium-dependent N-SMase activity via caspase-3 are interesting to future study to determine the relation between caspases and sphingolipid metabolites in NO-mediated signaling. Twit Text FOAVip Ceramide generation in nitric oxide-induced apoptosis Activation of magnesium-dependent neutral sphingomyelinase via caspase-3 ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=10187863 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=10187863 #FOAvip English Wikipedia <ref>{{Cite pmid|10187863}}</ref> Ceramide generation in nitric oxide-induced apoptosis Activation of magnesium-dependent neutral sphingomyelinase via caspase-3 #MMPMID10187863 Takeda Y; Tashima M; Takahashi A; Uchiyama T; Okazaki T J Biol Chem 1999[Apr]; 274 (15): 10654-60 PMID10187863show ga Sodium nitroprusside (SNP), a NO donor, has been recognized as an inducer of apoptosis in various cell lines. Here, we demonstrated the intracellular formation of ceramide, a lipid signal mediator, in SNP-induced apoptosis in human leukemia HL-60 cells and investigated the mechanisms of ceramide generation. The levels of intracellular ceramide increased to, at most, 160% of the control level in a time- and dose-dependent manner when the cells were treated with 1 mM SNP. SNP also decreased the sphingomyelin level to approximately 70% of the control level and increased magnesium-dependent neutral sphingomyelinase (N-SMase) activity to 160% of the control activity 2 h after treatment. Neither acid SMase nor magnesium-independent N-SMase was affected by SNP. Caspases are thought to be key enzymes in apoptotic cell death. Acetyl-Asp-Glu-Val-Asp-aldehyde, a synthetic tetrapeptide inhibitor of caspases, inhibited magnesiumdependent N-SMase, ceramide generation, and apoptosis. Moreover, recombinant purified caspase-3 increased magnesium-dependent N-SMase in a cell-free system. These results suggest that the findings that SNP increased ceramide generation and magnesium-dependent N-SMase activity via caspase-3 are interesting to future study to determine the relation between caspases and sphingolipid metabolites in NO-mediated signaling. |Apoptosis/*drug effects[MESH] |Caspase 3[MESH] |Caspases/*metabolism[MESH] |Ceramides/*metabolism[MESH] |Enzyme Activation[MESH] |Enzyme Inhibitors/pharmacology[MESH] |Flow Cytometry[MESH] |HL-60 Cells[MESH] |Humans[MESH] |Magnesium/*metabolism[MESH] |Nitric Oxide/*metabolism[MESH] |Nitroprusside/*pharmacology[MESH] |Oligopeptides/pharmacology[MESH] |Sphingomyelin Phosphodiesterase/*metabolism[MESH]Ceramide generation in nitric oxide-induced apoptosis Activation of (epmc).pdf DeepDyve Pubget Overpricing