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10.1046/j.1365-2362.1999.00429.x http://scihub22266oqcxt.onion/10.1046/j.1365-2362.1999.00429.x 10092994!ä!10092994 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Eur+J+Clin+Invest 1999 ; 29 (1): 83-92 Nephropedia Template TP {{tp|p=10092994|t=1999. Pulmonary surfactant protein A binds to Cryptococcus neoformans without promoting phagocytosis |pdf=|usr=}}{{10092994}} gab.com Text Pulmonary surfactant protein A binds to Cryptococcus neoformans without promoting phagocytosis JO: Eur J Clin Invest http://www.kidney.de/pget.php?&tw=1&pmid=10092994 #FOAvip BACKGROUND: Evidence is accumulating that the alveolar collecting surfactant protein A (SP-A) plays an important role in the first line of defence against infiltrating pathogenic micro-organisms and viruses. The ability of SP-A to facilitate the binding and uptake of acapsular Cryptococcus neoformans by monocyte-derived macrophages, human alveolar macrophages, monocytes and polymorphonuclear leucocytes was investigated. MATERIALS AND METHODS: Binding, competition and phagocytosis experiments were performed using a flow cytometry technique. RESULTS: SP-A bound to both the acapsular and the encapsulated form of C. neoformans in a concentration-dependent manner. SP-A showed a threefold better binding to the acapsular yeast: this binding was partly calcium dependent and could be inhibited by mannose (ID50 = 3 mmol L-1) and glucose (ID50 = 2.1 mmol L-1) but not by galactose (ID50 = 391 mmol L-1). SP-A did not function as an opsonin in phagocytosis of acapsular C. neoformans for any of the phagocytes studied. CONCLUSION: Our results indicate that SP-A binds in a concentration-dependent manner to both encapsulated and acapsular C. neoformans. Despite SP-A binding to the acapsular C. neoformans, phagocytosis by various phagocytes was not enhanced. Twit Text FOAVip Pulmonary surfactant protein A binds to Cryptococcus neoformans without promoting phagocytosis ????Eur J Clin Invest http://www.kidney.de/pget.php?&tw=1&pmid=10092994 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=10092994 #FOAvip English Wikipedia <ref>{{Cite pmid|10092994}}</ref> Pulmonary surfactant protein A binds to Cryptococcus neoformans without promoting phagocytosis #MMPMID10092994 Walenkamp AM; Verheul AF; Scharringa J; Hoepelman IM Eur J Clin Invest 1999[Jan]; 29 (1): 83-92 PMID10092994show ga BACKGROUND: Evidence is accumulating that the alveolar collecting surfactant protein A (SP-A) plays an important role in the first line of defence against infiltrating pathogenic micro-organisms and viruses. The ability of SP-A to facilitate the binding and uptake of acapsular Cryptococcus neoformans by monocyte-derived macrophages, human alveolar macrophages, monocytes and polymorphonuclear leucocytes was investigated. MATERIALS AND METHODS: Binding, competition and phagocytosis experiments were performed using a flow cytometry technique. RESULTS: SP-A bound to both the acapsular and the encapsulated form of C. neoformans in a concentration-dependent manner. SP-A showed a threefold better binding to the acapsular yeast: this binding was partly calcium dependent and could be inhibited by mannose (ID50 = 3 mmol L-1) and glucose (ID50 = 2.1 mmol L-1) but not by galactose (ID50 = 391 mmol L-1). SP-A did not function as an opsonin in phagocytosis of acapsular C. neoformans for any of the phagocytes studied. CONCLUSION: Our results indicate that SP-A binds in a concentration-dependent manner to both encapsulated and acapsular C. neoformans. Despite SP-A binding to the acapsular C. neoformans, phagocytosis by various phagocytes was not enhanced. |*Opsonin Proteins[MESH] |*Phagocytosis[MESH] |Animals[MESH] |Aspergillus fumigatus[MESH] |Binding Sites[MESH] |Calcium/pharmacology[MESH] |Carbohydrate Metabolism[MESH] |Cryptococcus neoformans/*metabolism[MESH] |Galactose/pharmacology[MESH] |Glucose/pharmacology[MESH] |Glycoproteins/metabolism[MESH] |Humans[MESH] |Influenza A virus[MESH] |Mannose/pharmacology[MESH] |Phagocytes[MESH] |Protein Binding/drug effects[MESH] |Proteolipids/*metabolism[MESH] |Pulmonary Surfactant-Associated Protein A[MESH] |Pulmonary Surfactant-Associated Protein D[MESH] |Pulmonary Surfactant-Associated Proteins[MESH] |Pulmonary Surfactants/*metabolism[MESH]Pulmonary surfactant protein A binds to Cryptococcus neoformans withou(epmc).pdf DeepDyve Pubget Overpricing