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10.1111/j.1469-7793.1999.639ab.x http://scihub22266oqcxt.onion/10.1111/j.1469-7793.1999.639ab.x 10066894!2269194!10066894     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Physiol 1999 ; 515 ( Pt 3) (Pt 3): 639-51 Nephropedia Template TP {{tp|p=10066894|t=1999. Kir2 1 encodes the inward rectifier potassium channel in rat arterial smooth muscle cells |pdf=|usr=}}{{10066894}} gab.com Text Kir2 1 encodes the inward rectifier potassium channel in rat arterial smooth muscle cells JO: J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=10066894 #FOAvip 1. The molecular nature of the strong inward rectifier K+ channel in vascular smooth muscle was explored by using isolated cell RT-PCR, cDNA cloning and expression techniques. 2. RT-PCR of RNA from single smooth muscle cells of rat cerebral (basilar), coronary and mesenteric arteries revealed transcripts for Kir2.1. Transcripts for Kir2.2 and Kir2.3 were not found. 3. Quantitative PCR analysis revealed significant differences in transcript levels of Kir2.1 between the different vascular preparations (n = 3; P < 0.05). A two-fold difference was detected between Kir2.1 mRNA and beta-actin mRNA in coronary arteries when compared with relative levels measured in mesenteric and basilar preparations. 4. Kir2.1 was cloned from rat mesenteric vascular smooth muscle cells and expressed in Xenopus oocytes. Currents were strongly inwardly rectifying and selective for K+. 5. The effect of extracellular Ba2+, Ca2+, Mg2+ and Cs2+ ions on cloned Kir2.1 channels expressed in Xenopus oocytes was examined. Ba2+ and Cs+ block were steeply voltage dependent, whereas block by external Ca2+ and Mg2+ exhibited little voltage dependence. The apparent half-block constants and voltage dependences for Ba2+, Cs+, Ca2+ and Mg2+ were very similar for inward rectifier K+ currents from native cells and cloned Kir2.1 channels expressed in oocytes. 6. Molecular studies demonstrate that Kir2.1 is the only member of the Kir2 channel subfamily present in vascular arterial smooth muscle cells. Expression of cloned Kir2.1 in Xenopus oocytes resulted in inward rectifier K+ currents that strongly resemble those that are observed in native vascular arterial smooth muscle cells. We conclude that Kir2.1 encodes for inward rectifier K+ channels in arterial smooth muscle. Twit Text FOAVip Kir2 1 encodes the inward rectifier potassium channel in rat arterial smooth muscle cells ????J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=10066894 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=10066894 #FOAvip English Wikipedia <ref>{{Cite pmid|10066894}}</ref> Kir2 1 encodes the inward rectifier potassium channel in rat arterial smooth muscle cells #MMPMID10066894 Bradley KK; Jaggar JH; Bonev AD; Heppner TJ; Flynn ER; Nelson MT; Horowitz B J Physiol 1999[Mar]; 515 ( Pt 3) (Pt 3): 639-51 PMID10066894show ga 1. The molecular nature of the strong inward rectifier K+ channel in vascular smooth muscle was explored by using isolated cell RT-PCR, cDNA cloning and expression techniques. 2. RT-PCR of RNA from single smooth muscle cells of rat cerebral (basilar), coronary and mesenteric arteries revealed transcripts for Kir2.1. Transcripts for Kir2.2 and Kir2.3 were not found. 3. Quantitative PCR analysis revealed significant differences in transcript levels of Kir2.1 between the different vascular preparations (n = 3; P < 0.05). A two-fold difference was detected between Kir2.1 mRNA and beta-actin mRNA in coronary arteries when compared with relative levels measured in mesenteric and basilar preparations. 4. Kir2.1 was cloned from rat mesenteric vascular smooth muscle cells and expressed in Xenopus oocytes. Currents were strongly inwardly rectifying and selective for K+. 5. The effect of extracellular Ba2+, Ca2+, Mg2+ and Cs2+ ions on cloned Kir2.1 channels expressed in Xenopus oocytes was examined. Ba2+ and Cs+ block were steeply voltage dependent, whereas block by external Ca2+ and Mg2+ exhibited little voltage dependence. The apparent half-block constants and voltage dependences for Ba2+, Cs+, Ca2+ and Mg2+ were very similar for inward rectifier K+ currents from native cells and cloned Kir2.1 channels expressed in oocytes. 6. Molecular studies demonstrate that Kir2.1 is the only member of the Kir2 channel subfamily present in vascular arterial smooth muscle cells. Expression of cloned Kir2.1 in Xenopus oocytes resulted in inward rectifier K+ currents that strongly resemble those that are observed in native vascular arterial smooth muscle cells. We conclude that Kir2.1 encodes for inward rectifier K+ channels in arterial smooth muscle. |*Potassium Channels, Inwardly Rectifying[MESH] |Animals[MESH] |Barium/pharmacology[MESH] |Basilar Artery/metabolism[MESH] |Cesium/pharmacology[MESH] |Cloning, Molecular[MESH] |Coronary Vessels/metabolism[MESH] |Membrane Potentials/drug effects[MESH] |Mesenteric Arteries/metabolism[MESH] |Muscle, Smooth, Vascular/*metabolism[MESH] |Oocytes/physiology[MESH] |Patch-Clamp Techniques[MESH] |Potassium Channels/drug effects/*genetics/*physiology[MESH] |Potassium/pharmacology[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH] |Recombinant Proteins/metabolism[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Transcription, Genetic[MESH]Kir2 1 encodes the inward rectifier potassium channel in rat arterial (epmc).pdf DeepDyve Pubget Overpricing