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2016 ; 11
(8
): e0161402
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eduSPIM: Light Sheet Microscopy in the Museum
#MMPMID27560188
Jahr W
; Schmid B
; Weber M
; Huisken J
PLoS One
2016[]; 11
(8
): e0161402
PMID27560188
show ga
LIGHT SHEET MICROSCOPY IN THE MUSEUM: Light sheet microscopy (or selective plane
illumination microscopy) is an important imaging technique in the life sciences.
At the same time, this technique is also ideally suited for community outreach
projects, because it produces visually appealing, highly dynamic images of living
organisms and its working principle can be understood with basic optics
knowledge. Still, the underlying concepts are widely unknown to the
non-scientific public. On the occasion of the UNESCO International Year of Light,
a technical museum in Dresden, Germany, launched a special, interactive
exhibition. We built a fully functional, educational selective plane illumination
microscope (eduSPIM) to demonstrate how developments in microscopy promote
discoveries in biology. DESIGN PRINCIPLES OF AN EDUCATIONAL LIGHT SHEET
MICROSCOPE: To maximize educational impact, we radically reduced a standard light
sheet microscope to its essential components without compromising functionality
and incorporated stringent safety concepts beyond those needed in the lab. Our
eduSPIM system features one illumination and one detection path and a sealed
sample chamber. We image fixed zebrafish embryos with fluorescent vasculature,
because the structure is meaningful to laymen and visualises the optical
principles of light sheet microscopy. Via a simplified interface, visitors
acquire fluorescence and transmission data simultaneously. THE EDUSPIM DESIGN IS
TAILORED EASILY TO FIT NUMEROUS APPLICATIONS: The universal concepts presented
here may also apply to other scientific approaches that are communicated to
laymen in interactive settings. The specific eduSPIM design is adapted easily for
various outreach and teaching activities. eduSPIM may even prove useful for labs
needing a simple SPIM. A detailed parts list and schematics to rebuild eduSPIM
are provided.
free
free
free
