Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25026365
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25026365
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Zebrafish
2014 ; 11
(5
): 434-46
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Zebrafish embryo model of Bartonella henselae infection
#MMPMID25026365
Lima A
; Cha BJ
; Amin J
; Smith LK
; Anderson B
Zebrafish
2014[Oct]; 11
(5
): 434-46
PMID25026365
show ga
Bartonella henselae (Bh) is an emerging zoonotic pathogen that has been
associated with a variety of human diseases, including bacillary angiomatosis
that is characterized by vasoproliferative tumor-like lesions on the skin of some
immunosuppressed individuals. The study of Bh pathogenesis has been limited to in
vitro cell culture systems due to the lack of an animal model. Therefore, we
wanted to investigate whether the zebrafish embryo could be used to model human
infection with Bh. Our data showed that Tg(fli1:egfp)(y1) zebrafish embryos
supported a sustained Bh infection for 7 days with >10-fold bacterial replication
when inoculated in the yolk sac. We showed that Bh recruited phagocytes to the
site of infection in the Tg(mpx:GFP)uwm1 embryos. Infected embryos showed
evidence of a Bh-induced angiogenic phenotype and an increase in the expression
of genes encoding pro-inflammatory factors and pro-angiogenic markers. However,
infection of zebrafish embryos with a deletion mutant in the major adhesin (BadA)
resulted in little or no bacterial replication and a diminished host response,
providing the first evidence that BadA is critical for in vivo infection. Thus,
the zebrafish embryo provides the first practical model of Bh infection that will
facilitate efforts to identify virulence factors and define molecular mechanisms
of Bh pathogenesis.
free
free
free
