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2017 ; 8
(9
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English Wikipedia
Zebrafish Xenograft: An Evolutionary Experiment in Tumour Biology
#MMPMID28872594
Wyatt RA
; Trieu NPV
; Crawford BD
Genes (Basel)
2017[Sep]; 8
(9
): ä PMID28872594
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Though the cancer research community has used mouse xenografts for decades more
than zebrafish xenografts, zebrafish have much to offer: they are cheap, easy to
work with, and the embryonic model is relatively easy to use in high-throughput
assays. Zebrafish can be imaged live, allowing us to observe cellular and
molecular processes in vivo in real time. Opponents dismiss the zebrafish model
due to the evolutionary distance between zebrafish and humans, as compared to
mice, but proponents argue for the zebrafish xenograft's superiority to cell
culture systems and its advantages in imaging. This review places the zebrafish
xenograft in the context of current views on cancer and gives an overview of how
several aspects of this evolutionary disease can be addressed in the zebrafish
model. Zebrafish are missing homologs of some human proteins and (of particular
interest) several members of the matrix metalloproteinase (MMP) family of
proteases, which are known for their importance in tumour biology. This review
draws attention to the implicit evolutionary experiment taking place when the
molecular ecology of the xenograft host is significantly different than that of
the donor.