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2014 ; 139
(12
): 3107-14
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Wide-field Raman imaging of dental lesions
#MMPMID24781363
Yang S
; Li B
; Akkus A
; Akkus O
; Lang L
Analyst
2014[Jun]; 139
(12
): 3107-14
PMID24781363
show ga
Detection of dental caries at the onset remains as a great challenge in
dentistry. Raman spectroscopy could be successfully applied towards detecting
caries since it is sensitive to the amount of Raman active mineral crystals, the
most abundant component of enamel. Effective diagnosis requires full examination
of a tooth surface via Raman mapping. Point-scan Raman mapping is not clinically
relevant (feasible) due to lengthy data acquisition time. In this work, a
wide-field Raman imaging system was assembled based on a high-sensitivity 2D CCD
camera for imaging the mineralization status of teeth with lesions. Wide-field
images indicated some lesions to be hypomineralized and others to be
hypermineralized. The observations of wide-field Raman imaging were in agreement
with point-scan Raman mapping. Therefore, sound enamel and lesions can be
discriminated by Raman imaging of the mineral content. In conclusion, wide-field
Raman imaging is a potentially useful tool for visualization of dental lesions in
the clinic.