Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27881844
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Sci+Rep
2016 ; 6
(ä): 37516
Nephropedia Template TP
Sci Rep
2016[Nov]; 6
(ä): 37516
PMID27881844
show ga
We present a wide-field imaging implementation of Fourier transform coherent
anti-Stokes Raman scattering (wide-field detected FT-CARS) microscopy capable of
acquiring high-contrast label-free but chemically specific images over the full
vibrational 'fingerprint' region, suitable for a large field of view. Rapid
resonant mechanical scanning of the illumination beam coupled with highly
sensitive, camera-based detection of the CARS signal allows for fast and direct
hyperspectral wide-field image acquisition, while minimizing sample damage.
Intrinsic to FT-CARS microscopy, the ability to control the range of time-delays
between pump and probe pulses allows for fine tuning of spectral resolution,
bandwidth and imaging speed while maintaining full duty cycle. We outline the
basic principles of wide-field detected FT-CARS microscopy and demonstrate how it
can be used as a sensitive optical probe for chemically specific Raman imaging.