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10.1038/srep37516

http://scihub22266oqcxt.onion/10.1038/srep37516
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C5121585!5121585 !27881844
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suck abstract from ncbi


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pmid27881844
      Sci+Rep 2016 ; 6 (ä): 37516
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  • Wide-Field Detected Fourier Transform CARS Microscopy #MMPMID27881844
  • Duarte AS ; Schnedermann C ; Kukura P
  • Sci Rep 2016[Nov]; 6 (ä): 37516 PMID27881844 show ga
  • We present a wide-field imaging implementation of Fourier transform coherent anti-Stokes Raman scattering (wide-field detected FT-CARS) microscopy capable of acquiring high-contrast label-free but chemically specific images over the full vibrational 'fingerprint' region, suitable for a large field of view. Rapid resonant mechanical scanning of the illumination beam coupled with highly sensitive, camera-based detection of the CARS signal allows for fast and direct hyperspectral wide-field image acquisition, while minimizing sample damage. Intrinsic to FT-CARS microscopy, the ability to control the range of time-delays between pump and probe pulses allows for fine tuning of spectral resolution, bandwidth and imaging speed while maintaining full duty cycle. We outline the basic principles of wide-field detected FT-CARS microscopy and demonstrate how it can be used as a sensitive optical probe for chemically specific Raman imaging.
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