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2016 ; 10
(ä): 31
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Whole Brain Imaging with Serial Two-Photon Tomography
#MMPMID27047350
Amato SP
; Pan F
; Schwartz J
; Ragan TM
Front Neuroanat
2016[]; 10
(ä): 31
PMID27047350
show ga
Imaging entire mouse brains at submicron resolution has historically been a
challenging undertaking and largely confined to the province of dedicated
atlasing initiatives. This has limited systematic investigations into important
areas of neuroscience, such as neural circuits, brain mapping and
neurodegeneration. In this article, we describe in detail Serial Two-Photon (STP)
tomography, a robust, reliable method for imaging entire brains with histological
detail. We provide examples of how the basic methodology can be extended to other
imaging modalities, such as Optical Coherence Tomography (OCT), in order to
provide unique contrast mechanisms. Furthermore, we provide a survey of the
research that STP tomography has enabled in the field of neuroscience, provide
examples of how this technology enables quantitative whole brain studies, and
discuss the current limitations of STP tomography-based approaches.