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10.3389/fnana.2016.00031

http://scihub22266oqcxt.onion/10.3389/fnana.2016.00031
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suck abstract from ncbi


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pmid27047350
      Front+Neuroanat 2016 ; 10 (ä): 31
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  • Whole Brain Imaging with Serial Two-Photon Tomography #MMPMID27047350
  • Amato SP ; Pan F ; Schwartz J ; Ragan TM
  • Front Neuroanat 2016[]; 10 (ä): 31 PMID27047350 show ga
  • Imaging entire mouse brains at submicron resolution has historically been a challenging undertaking and largely confined to the province of dedicated atlasing initiatives. This has limited systematic investigations into important areas of neuroscience, such as neural circuits, brain mapping and neurodegeneration. In this article, we describe in detail Serial Two-Photon (STP) tomography, a robust, reliable method for imaging entire brains with histological detail. We provide examples of how the basic methodology can be extended to other imaging modalities, such as Optical Coherence Tomography (OCT), in order to provide unique contrast mechanisms. Furthermore, we provide a survey of the research that STP tomography has enabled in the field of neuroscience, provide examples of how this technology enables quantitative whole brain studies, and discuss the current limitations of STP tomography-based approaches.
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