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2015 ; 31
(18
): 3078-80
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WGE: a CRISPR database for genome engineering
#MMPMID25979474
Hodgkins A
; Farne A
; Perera S
; Grego T
; Parry-Smith DJ
; Skarnes WC
; Iyer V
Bioinformatics
2015[Sep]; 31
(18
): 3078-80
PMID25979474
show ga
The rapid development of CRISPR-Cas9 mediated genome editing techniques has given
rise to a number of online and stand-alone tools to find and score CRISPR sites
for whole genomes. Here we describe the Wellcome Trust Sanger Institute Genome
Editing database (WGE), which uses novel methods to compute, visualize and select
optimal CRISPR sites in a genome browser environment. The WGE database currently
stores single and paired CRISPR sites and pre-calculated off-target information
for CRISPRs located in the mouse and human exomes. Scoring and display of
off-target sites is simple, and intuitive, and filters can be applied to identify
high-quality CRISPR sites rapidly. WGE also provides a tool for the design and
display of gene targeting vectors in the same genome browser, along with gene
models, protein translation and variation tracks. WGE is open, extensible and can
be set up to compute and present CRISPR sites for any genome. AVAILABILITY AND
IMPLEMENTATION: The WGE database is freely available at www.sanger.ac.uk/htgt/wge
CONTACT: : vvi@sanger.ac.uk or skarnes@sanger.ac.uk SUPPLEMENTARY INFORMATION:
Supplementary data are available at Bioinformatics online.
|*Databases, Factual
[MESH]
|*Gene Expression Regulation
[MESH]
|*Genetic Vectors
[MESH]
|*Genome
[MESH]
|Animals
[MESH]
|CRISPR-Cas Systems/*genetics
[MESH]
|Clustered Regularly Interspaced Short Palindromic Repeats/*genetics
[MESH]
free
free
free
