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2017 ; 7
(1
): 10539
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Visualizing Phonotactic Behavior of Female Frogs in Darkness
#MMPMID28874770
Aihara I
; Bishop PJ
; Ohmer MEB
; Awano H
; Mizumoto T
; Okuno HG
; Narins PM
; Hero JM
Sci Rep
2017[Sep]; 7
(1
): 10539
PMID28874770
show ga
Many animals use sounds produced by conspecifics for mate identification. Female
insects and anuran amphibians, for instance, use acoustic cues to localize,
orient toward and approach conspecific males prior to mating. Here we present a
novel technique that utilizes multiple, distributed sound-indication devices and
a miniature LED backpack to visualize and record the nocturnal phonotactic
approach of females of the Australian orange-eyed tree frog (Litoria chloris)
both in a laboratory arena and in the animal's natural habitat. Continuous
high-definition digital recording of the LED coordinates provides automatic
tracking of the female's position, and the illumination patterns of the
sound-indication devices allow us to discriminate multiple sound sources
including loudspeakers broadcasting calls as well as calls emitted by individual
male frogs. This innovative methodology is widely applicable for the study of
phonotaxis and spatial structures of acoustically communicating nocturnal
animals.