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10.3389/fpls.2018.00177 http://scihub22266oqcxt.onion/10.3389/fpls.2018.00177 C5820300!5820300 !29503656     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29503656 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Front+Plant+Sci 2018 ; 9 (ä): 177 Nephropedia Template TP {{tp|p=29503656 |t=2018. Visualization of the Nucleolus Using Ethynyl Uridine |pdf=|usr=}}{{29503656 }} gab.com Text Visualization of the Nucleolus Using Ethynyl Uridine JO: Front Plant Sci http://www.kidney.de/pget.php?&tw=1&pmid=29503656 #FOAvip Thanks to recent innovative methodologies, key cellular processes such as replication or transcription can be visualized directly in situ in intact tissues. Many studies use so-called click iT chemistry where nascent DNA can be tracked by 5-ethynyl-2'-deoxyuridine (EdU), and nascent RNA by 5-ethynyl uridine (EU). While the labeling of replicating DNA by EdU has already been well established and further exploited in plants, the use of EU to reveal nascent RNA has not been developed to such an extent. In this article, we present a protocol for labeling of nucleolar RNA transcripts using EU and show that EU effectively highlights the nucleolus. The method is advantageous, because the need to prepare transgenic plants expressing fluorescently tagged nucleolar components when the nucleolus has to be visualized can be avoided. Twit Text FOAVip Visualization of the Nucleolus Using Ethynyl Uridine ????Front Plant Sci http://www.kidney.de/pget.php?&tw=1&pmid=29503656 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29503656 #FOAvip English Wikipedia <ref>{{Cite pmid|29503656 }}</ref> Visualization of the Nucleolus Using Ethynyl Uridine #MMPMID29503656 Dvo?á?ková M ; Fajkus J Front Plant Sci 2018[]; 9 (ä): 177 PMID29503656 show ga Thanks to recent innovative methodologies, key cellular processes such as replication or transcription can be visualized directly in situ in intact tissues. Many studies use so-called click iT chemistry where nascent DNA can be tracked by 5-ethynyl-2'-deoxyuridine (EdU), and nascent RNA by 5-ethynyl uridine (EU). While the labeling of replicating DNA by EdU has already been well established and further exploited in plants, the use of EU to reveal nascent RNA has not been developed to such an extent. In this article, we present a protocol for labeling of nucleolar RNA transcripts using EU and show that EU effectively highlights the nucleolus. The method is advantageous, because the need to prepare transgenic plants expressing fluorescently tagged nucleolar components when the nucleolus has to be visualized can be avoided. äVisualization of the Nucleolus Using Ethynyl Uridine (epmc).pdf DeepDyve Pubget Overpricing