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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Am+Soc+Nephrol
2015 ; 26
(11
): 2731-40
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Visualization of Calcium Dynamics in Kidney Proximal Tubules
#MMPMID25788535
Szebényi K
; Füredi A
; Kolacsek O
; Csohány R
; Prókai Á
; Kis-Petik K
; Szabó A
; B?sze Z
; Bender B
; Tóvári J
; Enyedi Á
; Orbán TI
; Apáti Á
; Sarkadi B
J Am Soc Nephrol
2015[Nov]; 26
(11
): 2731-40
PMID25788535
show ga
Intrarenal changes in cytoplasmic calcium levels have a key role in determining
pathologic and pharmacologic responses in major kidney diseases. However,
cell-specific delivery of calcium-sensitive probes in vivo remains problematic.
We generated a transgenic rat stably expressing the green fluorescent
protein-calmodulin-based genetically encoded calcium indicator (GCaMP2)
predominantly in the kidney proximal tubules. The transposon-based method used
allowed the generation of homozygous transgenic rats containing one copy of the
transgene per allele with a defined insertion pattern, without genetic or
phenotypic alterations. We applied in vitro confocal and in vivo two-photon
microscopy to examine basal calcium levels and ligand- and drug-induced
alterations in these levels in proximal tubular epithelial cells. Notably, renal
ischemia induced a transient increase in cellular calcium, and reperfusion
resulted in a secondary calcium load, which was significantly decreased by
systemic administration of specific blockers of the angiotensin receptor and the
Na-Ca exchanger. The parallel examination of in vivo cellular calcium dynamics
and renal circulation by fluorescent probes opens new possibilities for
physiologic and pharmacologic investigations.
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