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2013 ; 16
(4
): 468-78
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Viral pathogen discovery
#MMPMID23725672
Chiu CY
Curr Opin Microbiol
2013[Aug]; 16
(4
): 468-78
PMID23725672
show ga
Viral pathogen discovery is of critical importance to clinical microbiology,
infectious diseases, and public health. Genomic approaches for pathogen
discovery, including consensus polymerase chain reaction (PCR), microarrays, and
unbiased next-generation sequencing (NGS), have the capacity to comprehensively
identify novel microbes present in clinical samples. Although numerous challenges
remain to be addressed, including the bioinformatics analysis and interpretation
of large datasets, these technologies have been successful in rapidly identifying
emerging outbreak threats, screening vaccines and other biological products for
microbial contamination, and discovering novel viruses associated with both acute
and chronic illnesses. Downstream studies such as genome assembly, epidemiologic
screening, and a culture system or animal model of infection are necessary to
establish an association of a candidate pathogen with disease.