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10.1371/journal.pone.0118192 http://scihub22266oqcxt.onion/10.1371/journal.pone.0118192 C4370828!4370828 !25799103     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25799103 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2015 ; 10 (3 ): e0118192 Nephropedia Template TP {{tp|p=25799103 |t=2015. VDJSeq-Solver: in silico V(D)J recombination detection tool |pdf=|usr=}}{{25799103 }} gab.com Text VDJSeq-Solver: in silico V(D)J recombination detection tool JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25799103 #FOAvip In this paper we present VDJSeq-Solver, a methodology and tool to identify clonal lymphocyte populations from paired-end RNA Sequencing reads derived from the sequencing of mRNA neoplastic cells. The tool detects the main clone that characterises the tissue of interest by recognizing the most abundant V(D)J rearrangement among the existing ones in the sample under study. The exact sequence of the clone identified is capable of accounting for the modifications introduced by the enzymatic processes. The proposed tool overcomes limitations of currently available lymphocyte rearrangements recognition methods, working on a single sequence at a time, that are not applicable to high-throughput sequencing data. In this work, VDJSeq-Solver has been applied to correctly detect the main clone and identify its sequence on five Mantle Cell Lymphoma samples; then the tool has been tested on twelve Diffuse Large B-Cell Lymphoma samples. In order to comply with the privacy, ethics and intellectual property policies of the University Hospital and the University of Verona, data is available upon request to supporto.utenti@ateneo.univr.it after signing a mandatory Materials Transfer Agreement. VDJSeq-Solver JAVA/Perl/Bash software implementation is free and available at http://eda.polito.it/VDJSeq-Solver/. Twit Text FOAVip VDJSeq-Solver: in silico V(D)J recombination detection tool ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25799103 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25799103 #FOAvip English Wikipedia <ref>{{Cite pmid|25799103 }}</ref> VDJSeq-Solver: in silico V(D)J recombination detection tool #MMPMID25799103 Paciello G ; Acquaviva A ; Pighi C ; Ferrarini A ; Macii E ; Zamo' A ; Ficarra E PLoS One 2015[]; 10 (3 ): e0118192 PMID25799103 show ga In this paper we present VDJSeq-Solver, a methodology and tool to identify clonal lymphocyte populations from paired-end RNA Sequencing reads derived from the sequencing of mRNA neoplastic cells. The tool detects the main clone that characterises the tissue of interest by recognizing the most abundant V(D)J rearrangement among the existing ones in the sample under study. The exact sequence of the clone identified is capable of accounting for the modifications introduced by the enzymatic processes. The proposed tool overcomes limitations of currently available lymphocyte rearrangements recognition methods, working on a single sequence at a time, that are not applicable to high-throughput sequencing data. In this work, VDJSeq-Solver has been applied to correctly detect the main clone and identify its sequence on five Mantle Cell Lymphoma samples; then the tool has been tested on twelve Diffuse Large B-Cell Lymphoma samples. In order to comply with the privacy, ethics and intellectual property policies of the University Hospital and the University of Verona, data is available upon request to supporto.utenti@ateneo.univr.it after signing a mandatory Materials Transfer Agreement. VDJSeq-Solver JAVA/Perl/Bash software implementation is free and available at http://eda.polito.it/VDJSeq-Solver/. |*Computer Simulation [MESH] |*Genes, Immunoglobulin [MESH] |*Software [MESH] |Algorithms [MESH] |Clone Cells [MESH] |High-Throughput Nucleotide Sequencing/methods [MESH] |Humans [MESH] |Lymphoma, Large B-Cell, Diffuse/*diagnosis/genetics [MESH] |Lymphoma, Mantle-Cell/*diagnosis/genetics [MESH] |Polymerase Chain Reaction [MESH] |Sequence Analysis, RNA/methods [MESH]VDJSeq-Solver: in silico V(D)J recombination detection tool (epmc).pdf DeepDyve Pubget Overpricing