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2017 ; 22
(3
): 36013
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Ultraminiature optical design for multispectral fluorescence imaging endoscopes
#MMPMID28334332
Tate TH
; Keenan M
; Black J
; Utzinger U
; Barton JK
J Biomed Opt
2017[Mar]; 22
(3
): 36013
PMID28334332
show ga
A miniature wide-field multispectral endoscopic imaging system was developed
enabling reflectance and fluorescence imaging over a broad wavelength range. At
0.8-mm diameter, the endoscope can be utilized for natural orifice imaging in
small lumens such as the fallopian tubes. Five lasers from 250 to 642 nm are
coupled into a 125 - ? m diameter multimode fiber and transmitted to the
endoscope distal tip for illumination. Ultraviolet and blue wavelengths excite
endogenous fluorophores, which can provide differential fluorescence emission
images for health and disease. Visible wavelengths provide reflectance images
that can be combined for pseudo-white-light imaging and navigation. Imaging is
performed by a 300 - ? m diameter three-element lens system connected to a
3000-element fiber. The lens system was designed for a 70-deg full field of view,
working distance from 3 mm to infinity, and 40% contrast at the Nyquist cutoff of
the fiber bundle. Measured performance characteristics are near design goals. The
endoscope was utilized to obtain example monochromatic, pseudo-white-light, and
composite fluorescence images of phantoms and porcine reproductive tract. This
work shows the feasibility of packaging a highly capable multispectral
fluorescence imaging system into a miniature endoscopic system that may have
applications in early detection of cancer.