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10.1364/OE.23.013833

http://scihub22266oqcxt.onion/10.1364/OE.23.013833
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C4523368!4523368 !26072755
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suck abstract from ncbi


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pmid26072755
      Opt+Express 2015 ; 23 (11 ): 13833-47
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  • Ultra-large field-of-view two-photon microscopy #MMPMID26072755
  • Tsai PS ; Mateo C ; Field JJ ; Schaffer CB ; Anderson ME ; Kleinfeld D
  • Opt Express 2015[Jun]; 23 (11 ): 13833-47 PMID26072755 show ga
  • We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across an 8 mm by 10 mm field. The lateral resolution is approximately 1 µm and the maximum scan speed is 5 mm/ms. The scan pathway employs large diameter compound lenses to minimize aberrations and performs near theoretical limits. We demonstrate the special utility of the microscope by recording resting-state vasomotion across both hemispheres of the murine brain through a transcranial window and by imaging histological sections without the need to stitch.
  • |Animals [MESH]
  • |Cerebral Cortex/blood supply [MESH]
  • |Equipment Design [MESH]
  • |Imaging, Three-Dimensional [MESH]
  • |Mice [MESH]
  • |Microscopy, Fluorescence, Multiphoton/instrumentation/*methods [MESH]
  • |Optical Phenomena [MESH]


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