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2010 ; 32
(3
): 297-304
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Two-photon microscopy in pulmonary research
#MMPMID20589501
Nava RG
; Li W
; Gelman AE
; Krupnick AS
; Miller MJ
; Kreisel D
Semin Immunopathol
2010[Sep]; 32
(3
): 297-304
PMID20589501
show ga
As the lung is constantly exposed to both innocuous and potentially noxious
antigens, a thorough understanding of both innate and adaptive immune responses
in this organ is of the essence. Imaging modalities such as magnetic resonance
imaging, positron emission tomography, and confocal microscopy have expanded our
knowledge about various molecular processes and cellular responses in the lung.
Two-photon microscopy has evolved into a powerful tool to observe cellular
interactions in real time and has markedly expanded our understanding of the
immune system. Recently, two-photon microscopy has also been utilized to image
the murine lung. As immune responses in the lung differ from those in other
non-lymphoid tissues, this technique holds great promise to advance our knowledge
of the biology that underlies a wide spectrum of pulmonary diseases.