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10.1038/srep23866 http://scihub22266oqcxt.onion/10.1038/srep23866 C4814926!4814926 !27029524     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27029524 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27029524 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2016 ; 6 (ä): 23866 Nephropedia Template TP {{tp|p=27029524 |t=2016. Two-photon excited photoconversion of cyanine-based dyes |pdf=|usr=}}{{27029524 }} gab.com Text Two-photon excited photoconversion of cyanine-based dyes JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27029524 #FOAvip The advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting, and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue. Twit Text FOAVip Two-photon excited photoconversion of cyanine-based dyes ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27029524 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27029524 #FOAvip English Wikipedia <ref>{{Cite pmid|27029524 }}</ref> Two-photon excited photoconversion of cyanine-based dyes #MMPMID27029524 Kwok SJ ; Choi M ; Bhayana B ; Zhang X ; Ran C ; Yun SH Sci Rep 2016[Mar]; 6 (ä): 23866 PMID27029524 show ga The advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting, and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue. |Animals [MESH] |Carbocyanines/*chemistry/metabolism [MESH] |Cell Line [MESH] |Cell Tracking/*methods [MESH] |Erythrocytes/*metabolism/ultrastructure [MESH] |Fluorescent Dyes/*chemistry/metabolism [MESH] |HeLa Cells [MESH] |Humans [MESH] |Jurkat Cells [MESH] |Light [MESH] |Macrophages/*metabolism/ultrastructure [MESH] |Mice [MESH] |Microscopy, Fluorescence, Multiphoton [MESH] |Photochemical Processes [MESH]Two-photon excited photoconversion of cyanine-based dyes (epmc).pdf DeepDyve Pubget Overpricing