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2016 ; 2
(1
): 21-32
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Tumor-Initiating Cells: Emerging Biophysical Methods of Isolation
#MMPMID27141429
Cermeño EA
; García AJ
Curr Stem Cell Rep
2016[Mar]; 2
(1
): 21-32
PMID27141429
show ga
The discovery and subsequent isolation of tumor-initiating cells (TICs), a small
population of highly tumorigenic and drug-resistant cancer cells also called
cancer stem cells (CSCs), have revolutionized our understanding of cancer. TICs
are isolated using various methodologies, including selection of surface marker
expression, ALDH activity, suspension culture, and chemotherapy/drug resistance.
These methods have several drawbacks, including their variability, lack of
robustness and scalability, and low specificity. Alternative methods of
purification take advantage of biophysical properties of TICs including their
adhesion and stiffness. This review will provide a brief overview of TIC biology
as well as review the most important methods of TIC isolation with a focus on
biophysical methods of TIC purification.