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10.1681/ASN.2013101143 http://scihub22266oqcxt.onion/10.1681/ASN.2013101143 C4147988!4147988 !24652793     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24652793 &cmd=llinks): Failed to open stream: HTTP request failed! 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Translational profiles of medullary myofibroblasts during kidney fibrosis |pdf=|usr=}}{{24652793 }} gab.com Text Translational profiles of medullary myofibroblasts during kidney fibrosis JO: J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=24652793 #FOAvip Myofibroblasts secrete matrix during chronic injury, and their ablation ameliorates fibrosis. Development of new biomarkers and therapies for CKD will be aided by a detailed analysis of myofibroblast gene expression during the early stages of fibrosis. However, dissociating myofibroblasts from fibrotic kidney is challenging. We therefore adapted translational ribosome affinity purification (TRAP) to isolate and profile mRNA from myofibroblasts and their precursors during kidney fibrosis. We generated and characterized a transgenic mouse expressing an enhanced green fluorescent protein (eGFP)-tagged L10a ribosomal subunit protein under control of the collagen1?1 promoter. We developed a one-step procedure for isolation of polysomal RNA from collagen1?1-eGFPL10a mice subject to unilateral ureteral obstruction and analyzed and validated the resulting transcriptional profiles. Pathway analysis revealed strong gene signatures for cell proliferation, migration, and shape change. Numerous novel genes and candidate biomarkers were upregulated during fibrosis, specifically in myofibroblasts, and we validated these results by quantitative PCR, in situ, and Western blot analysis. This study provides a comprehensive analysis of early myofibroblast gene expression during kidney fibrosis and introduces a new technique for cell-specific polysomal mRNA isolation in kidney injury models that is suited for RNA-sequencing technologies. Twit Text FOAVip Translational profiles of medullary myofibroblasts during kidney fibrosis ????J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=24652793 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24652793 #FOAvip English Wikipedia <ref>{{Cite pmid|24652793 }}</ref> Translational profiles of medullary myofibroblasts during kidney fibrosis #MMPMID24652793 Grgic I ; Krautzberger AM ; Hofmeister A ; Lalli M ; DiRocco DP ; Fleig SV ; Liu J ; Duffield JS ; McMahon AP ; Aronow B ; Humphreys BD J Am Soc Nephrol 2014[Sep]; 25 (9 ): 1979-90 PMID24652793 show ga Myofibroblasts secrete matrix during chronic injury, and their ablation ameliorates fibrosis. Development of new biomarkers and therapies for CKD will be aided by a detailed analysis of myofibroblast gene expression during the early stages of fibrosis. However, dissociating myofibroblasts from fibrotic kidney is challenging. We therefore adapted translational ribosome affinity purification (TRAP) to isolate and profile mRNA from myofibroblasts and their precursors during kidney fibrosis. We generated and characterized a transgenic mouse expressing an enhanced green fluorescent protein (eGFP)-tagged L10a ribosomal subunit protein under control of the collagen1?1 promoter. We developed a one-step procedure for isolation of polysomal RNA from collagen1?1-eGFPL10a mice subject to unilateral ureteral obstruction and analyzed and validated the resulting transcriptional profiles. Pathway analysis revealed strong gene signatures for cell proliferation, migration, and shape change. Numerous novel genes and candidate biomarkers were upregulated during fibrosis, specifically in myofibroblasts, and we validated these results by quantitative PCR, in situ, and Western blot analysis. This study provides a comprehensive analysis of early myofibroblast gene expression during kidney fibrosis and introduces a new technique for cell-specific polysomal mRNA isolation in kidney injury models that is suited for RNA-sequencing technologies. |Animals [MESH] |Collagen Type I, alpha 1 Chain [MESH] |Collagen Type I/genetics [MESH] |Disease Models, Animal [MESH] |Fibrosis [MESH] |Gene Expression Profiling/methods [MESH] |Green Fluorescent Proteins/genetics/metabolism [MESH] |Kidney/injuries/*metabolism/*pathology [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Mice, Inbred DBA [MESH] |Mice, Transgenic [MESH] |Myofibroblasts/*metabolism/*pathology [MESH] |Oligonucleotide Array Sequence Analysis [MESH] |RNA, Messenger/genetics/metabolism [MESH] |Recombinant Fusion Proteins/genetics/metabolism [MESH] |Ribosomal Protein L10 [MESH] |Ribosomal Proteins/genetics/metabolism [MESH] |Up-Regulation [MESH]Translational profiles of medullary myofibroblasts during kidney fibro(epmc).pdf DeepDyve Pubget Overpricing