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2013 ; 42
(ä): 583-604
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Torque measurement at the single-molecule level
#MMPMID23541162
Forth S
; Sheinin MY
; Inman J
; Wang MD
Annu Rev Biophys
2013[]; 42
(ä): 583-604
PMID23541162
show ga
Methods for exerting and measuring forces on single molecules have revolutionized
the study of the physics of biology. However, it is often the case that
biological processes involve rotation or torque generation, and these parameters
have been more difficult to access experimentally. Recent advances in the
single-molecule field have led to the development of techniques that add the
capability of torque measurement. By combining force, displacement, torque, and
rotational data, a more comprehensive description of the mechanics of a
biomolecule can be achieved. In this review, we highlight a number of biological
processes for which torque plays a key mechanical role. We describe the various
techniques that have been developed to directly probe the torque experienced by a
single molecule, and detail a variety of measurements made to date using these
new technologies. We conclude by discussing a number of open questions and
propose systems of study that would be well suited for analysis with torsional
measurement techniques.