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10.1002/0471143030.cb1716s64 http://scihub22266oqcxt.onion/10.1002/0471143030.cb1716s64 C4181358!4181358 !25181301     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25181301 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Curr+Protoc+Cell+Biol 2014 ; 64 (ä): 17.16.1-20 Nephropedia Template TP {{tp|p=25181301 |t=2014. Tools for controlling protein interactions using light |pdf=|usr=}}{{25181301 }} gab.com Text Tools for controlling protein interactions using light JO: Curr Protoc Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=25181301 #FOAvip Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers', are emerging as new tools for experimental biology. In recent years, numerous new and versatile dimerizer systems have been developed. Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters. We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light. Additionally, we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments. Twit Text FOAVip Tools for controlling protein interactions using light ????Curr Protoc Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=25181301 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25181301 #FOAvip English Wikipedia <ref>{{Cite pmid|25181301 }}</ref> Tools for controlling protein interactions using light #MMPMID25181301 Tucker CL ; Vrana JD ; Kennedy MJ Curr Protoc Cell Biol 2014[Sep]; 64 (ä): 17.16.1-20 PMID25181301 show ga Genetically encoded actuators that allow control of protein-protein interactions using light, termed 'optical dimerizers', are emerging as new tools for experimental biology. In recent years, numerous new and versatile dimerizer systems have been developed. Here we discuss the design of optical dimerizer experiments, including choice of a dimerizer system, photoexcitation sources, and the coordinate use of imaging reporters. We provide detailed protocols for experiments using two dimerization systems we previously developed, CRY2/CIB and UVR8/UVR8, for use in controlling transcription, protein localization, and protein secretion using light. Additionally, we provide instructions and software for constructing a pulse-controlled LED device for use in experiments requiring extended light treatments. |*Light [MESH] |*Protein Multimerization [MESH] |Animals [MESH] |Arabidopsis Proteins/*chemistry/genetics [MESH] |COS Cells [MESH] |Chlorocebus aethiops [MESH] |Chromosomal Proteins, Non-Histone/*chemistry/genetics [MESH] |Cryptochromes/*chemistry/genetics [MESH] |HeLa Cells [MESH]Tools for controlling protein interactions using light (epmc).pdf DeepDyve Pubget Overpricing