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10.1089/ten.TEA.2015.0060 http://scihub22266oqcxt.onion/10.1089/ten.TEA.2015.0060 C4652181!4652181 !26414101     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26414101 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Tissue+Eng+Part+A 2015 ; 21 (21-22 ): 2673-9 Nephropedia Template TP {{tp|p=26414101 |t=2015. Tissue-Engineered Microvasculature to Reperfuse Isolated Renal Glomeruli |pdf=|usr=}}{{26414101 }} gab.com Text Tissue-Engineered Microvasculature to Reperfuse Isolated Renal Glomeruli JO: Tissue Eng Part A http://www.kidney.de/pget.php?&tw=1&pmid=26414101 #FOAvip Kidney transplantation is often the most effective therapy for end-stage renal disease, but there are not enough donor organs to meet the rising demand. Tissue engineering of kidneys is a potential solution to this organ shortage. Achieving microvascular perfusion has been a major barrier to engineering tissues beyond thin muscularized sheets such as the bladder wall. Our laboratory has previously reported that human umbilical vein endothelial cells (ECs) transduced with the antiapoptotic protein Bcl-2 will spontaneously organize into perfused microvessels within type I collagen gels when implanted in immunodeficient mice. To test if this system can be used to perfuse more complex structures, we combined Bcl-2-transduced ECs (Bcl-2-ECs) with renal glomeruli, the specialized vascular filtration units of the kidney. Microdissected green fluorescent protein-expressing rat glomeruli suspended in type I collagen gels were implanted within immunodeficient mice with or without the inclusion of Bcl-2-ECs. Survival of rat glomeruli was enhanced by coimplantation with Bcl-2-ECs. Intravital rhodamine dextran injections demonstrated that surviving glomeruli were perfused through Bcl-2-EC-derived microvessels. Perfused glomeruli maintained podocin staining, but transmission electron microscopy revealed endothelial swelling and podocyte foot process effacement. Anastomosis of microvessels derived from Bcl-2-ECs with glomerular capillaries provides proof of concept that self-assembled microvessels can perfuse specialized organ structures such as glomeruli, but that perfusion alone may be insufficient to maintain normal structure. Twit Text FOAVip Tissue-Engineered Microvasculature to Reperfuse Isolated Renal Glomeruli ????Tissue Eng Part A http://www.kidney.de/pget.php?&tw=1&pmid=26414101 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26414101 #FOAvip English Wikipedia <ref>{{Cite pmid|26414101 }}</ref> Tissue-Engineered Microvasculature to Reperfuse Isolated Renal Glomeruli #MMPMID26414101 Chang WG ; Fornoni A ; Tietjen G ; Mendez JJ ; Niklason LE ; Saltzman WM ; Pober JS Tissue Eng Part A 2015[Nov]; 21 (21-22 ): 2673-9 PMID26414101 show ga Kidney transplantation is often the most effective therapy for end-stage renal disease, but there are not enough donor organs to meet the rising demand. Tissue engineering of kidneys is a potential solution to this organ shortage. Achieving microvascular perfusion has been a major barrier to engineering tissues beyond thin muscularized sheets such as the bladder wall. Our laboratory has previously reported that human umbilical vein endothelial cells (ECs) transduced with the antiapoptotic protein Bcl-2 will spontaneously organize into perfused microvessels within type I collagen gels when implanted in immunodeficient mice. To test if this system can be used to perfuse more complex structures, we combined Bcl-2-transduced ECs (Bcl-2-ECs) with renal glomeruli, the specialized vascular filtration units of the kidney. Microdissected green fluorescent protein-expressing rat glomeruli suspended in type I collagen gels were implanted within immunodeficient mice with or without the inclusion of Bcl-2-ECs. Survival of rat glomeruli was enhanced by coimplantation with Bcl-2-ECs. Intravital rhodamine dextran injections demonstrated that surviving glomeruli were perfused through Bcl-2-EC-derived microvessels. Perfused glomeruli maintained podocin staining, but transmission electron microscopy revealed endothelial swelling and podocyte foot process effacement. Anastomosis of microvessels derived from Bcl-2-ECs with glomerular capillaries provides proof of concept that self-assembled microvessels can perfuse specialized organ structures such as glomeruli, but that perfusion alone may be insufficient to maintain normal structure. |Animals [MESH] |Cells, Cultured [MESH] |Endothelial Cells/*cytology/physiology/*transplantation [MESH] |Female [MESH] |Humans [MESH] |In Vitro Techniques [MESH] |Kidney Glomerulus/*blood supply/physiology/surgery [MESH] |Mice, Inbred C57BL [MESH] |Microvessels/cytology/*growth & development [MESH] |Rats [MESH] |Rats, Transgenic [MESH] |Reperfusion/*methods [MESH] |Tissue Engineering/*methods [MESH]Tissue-Engineered Microvasculature to Reperfuse Isolated Renal Glomeru(epmc).pdf DeepDyve Pubget Overpricing