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10.1038/srep12529 http://scihub22266oqcxt.onion/10.1038/srep12529 C4515588!4515588 !26211729     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26211729 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 12529 Nephropedia Template TP {{tp|p=26211729 |t=2015. Time-lapse Raman imaging of osteoblast differentiation |pdf=|usr=}}{{26211729 }} gab.com Text Time-lapse Raman imaging of osteoblast differentiation JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26211729 #FOAvip Osteoblastic mineralization occurs during the early stages of bone formation. During this mineralization, hydroxyapatite (HA), a major component of bone, is synthesized, generating hard tissue. Many of the mechanisms driving biomineralization remain unclear because the traditional biochemical assays used to investigate them are destructive techniques incompatible with viable cells. To determine the temporal changes in mineralization-related biomolecules at mineralization spots, we performed time-lapse Raman imaging of mouse osteoblasts at a subcellular resolution throughout the mineralization process. Raman imaging enabled us to analyze the dynamics of the related biomolecules at mineralization spots throughout the entire process of mineralization. Here, we stimulated KUSA-A1 cells to differentiate into osteoblasts and conducted time-lapse Raman imaging on them every 4?hours for 24?hours, beginning 5 days after the stimulation. The HA and cytochrome c Raman bands were used as markers for osteoblastic mineralization and apoptosis. From the Raman images successfully acquired throughout the mineralization process, we found that ?-carotene acts as a biomarker that indicates the initiation of osteoblastic mineralization. A fluctuation of cytochrome c concentration, which indicates cell apoptosis, was also observed during mineralization. We expect time-lapse Raman imaging to help us to further elucidate osteoblastic mineralization mechanisms that have previously been unobservable. Twit Text FOAVip Time-lapse Raman imaging of osteoblast differentiation ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26211729 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26211729 #FOAvip English Wikipedia <ref>{{Cite pmid|26211729 }}</ref> Time-lapse Raman imaging of osteoblast differentiation #MMPMID26211729 Hashimoto A ; Yamaguchi Y ; Chiu LD ; Morimoto C ; Fujita K ; Takedachi M ; Kawata S ; Murakami S ; Tamiya E Sci Rep 2015[Jul]; 5 (ä): 12529 PMID26211729 show ga Osteoblastic mineralization occurs during the early stages of bone formation. During this mineralization, hydroxyapatite (HA), a major component of bone, is synthesized, generating hard tissue. Many of the mechanisms driving biomineralization remain unclear because the traditional biochemical assays used to investigate them are destructive techniques incompatible with viable cells. To determine the temporal changes in mineralization-related biomolecules at mineralization spots, we performed time-lapse Raman imaging of mouse osteoblasts at a subcellular resolution throughout the mineralization process. Raman imaging enabled us to analyze the dynamics of the related biomolecules at mineralization spots throughout the entire process of mineralization. Here, we stimulated KUSA-A1 cells to differentiate into osteoblasts and conducted time-lapse Raman imaging on them every 4?hours for 24?hours, beginning 5 days after the stimulation. The HA and cytochrome c Raman bands were used as markers for osteoblastic mineralization and apoptosis. From the Raman images successfully acquired throughout the mineralization process, we found that ?-carotene acts as a biomarker that indicates the initiation of osteoblastic mineralization. A fluctuation of cytochrome c concentration, which indicates cell apoptosis, was also observed during mineralization. We expect time-lapse Raman imaging to help us to further elucidate osteoblastic mineralization mechanisms that have previously been unobservable. |Animals [MESH] |Calcification, Physiologic/*physiology [MESH] |Cell Differentiation/physiology [MESH] |Cell Line [MESH] |Cytochromes c/metabolism [MESH] |Mice [MESH] |Microscopy/*methods [MESH] |Osteoblasts/*cytology/*physiology [MESH] |Spectrum Analysis, Raman/*methods [MESH] |Time-Lapse Imaging/*methods [MESH]Time-lapse Raman imaging of osteoblast differentiation (epmc).pdf DeepDyve Pubget Overpricing