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10.1007/s00216-015-9018-2 http://scihub22266oqcxt.onion/10.1007/s00216-015-9018-2 C4636921!4636921 !26342315     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26342315 &cmd=llinks): Failed to open stream: HTTP request failed! 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Theoretical limitations of quantification for noncompetitive sandwich immunoassays |pdf=|usr=}}{{26342315 }} gab.com Text Theoretical limitations of quantification for noncompetitive sandwich immunoassays JO: Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=26342315 #FOAvip Immunoassays exploit the highly selective interaction between antibodies and antigens to provide a vital method for biomolecule detection at low concentrations. Developers and practitioners of immunoassays have long known that non-specific binding often restricts immunoassay limits of quantification (LOQs). Aside from non-specific binding, most efforts by analytical chemists to reduce the LOQ for these techniques have focused on improving the signal amplification methods and minimizing the limitations of the detection system. However, with detection technology now capable of sensing single-fluorescence molecules, this approach is unlikely to lead to dramatic improvements in the future. Here, fundamental interactions based on the law of mass action are analytically connected to signal generation, replacing the four- and five-parameter fittings commercially used to approximate sigmoidal immunoassay curves and allowing quantitative consideration of non-specific binding and statistical limitations in order to understand the ultimate detection capabilities of immunoassays. The restrictions imposed on limits of quantification by instrumental noise, non-specific binding, and counting statistics are discussed based on equilibrium relations for a sandwich immunoassay. Understanding the maximal capabilities of immunoassays for each of these regimes can greatly assist in the development and evaluation of immunoassay platforms. While many studies suggest that single molecule detection is possible through immunoassay techniques, here, it is demonstrated that the fundamental limit of quantification (precision of 10 % or better) for an immunoassay is approximately 131 molecules and this limit is based on fundamental and unavoidable statistical limitations. Twit Text FOAVip Theoretical limitations of quantification for noncompetitive sandwich immunoassays ????Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=26342315 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26342315 #FOAvip English Wikipedia <ref>{{Cite pmid|26342315 }}</ref> Theoretical limitations of quantification for noncompetitive sandwich immunoassays #MMPMID26342315 Woolley CF ; Hayes MA ; Mahanti P ; Douglass Gilman S ; Taylor T Anal Bioanal Chem 2015[Nov]; 407 (28 ): 8605-15 PMID26342315 show ga Immunoassays exploit the highly selective interaction between antibodies and antigens to provide a vital method for biomolecule detection at low concentrations. Developers and practitioners of immunoassays have long known that non-specific binding often restricts immunoassay limits of quantification (LOQs). Aside from non-specific binding, most efforts by analytical chemists to reduce the LOQ for these techniques have focused on improving the signal amplification methods and minimizing the limitations of the detection system. However, with detection technology now capable of sensing single-fluorescence molecules, this approach is unlikely to lead to dramatic improvements in the future. Here, fundamental interactions based on the law of mass action are analytically connected to signal generation, replacing the four- and five-parameter fittings commercially used to approximate sigmoidal immunoassay curves and allowing quantitative consideration of non-specific binding and statistical limitations in order to understand the ultimate detection capabilities of immunoassays. The restrictions imposed on limits of quantification by instrumental noise, non-specific binding, and counting statistics are discussed based on equilibrium relations for a sandwich immunoassay. Understanding the maximal capabilities of immunoassays for each of these regimes can greatly assist in the development and evaluation of immunoassay platforms. While many studies suggest that single molecule detection is possible through immunoassay techniques, here, it is demonstrated that the fundamental limit of quantification (precision of 10 % or better) for an immunoassay is approximately 131 molecules and this limit is based on fundamental and unavoidable statistical limitations. |*Limit of Detection [MESH] |Antibodies/*chemistry [MESH] |Antigens/*chemistry [MESH] |Humans [MESH] |Immunoassay/*statistics & numerical data [MESH] |Multivariate Analysis [MESH] |Protein Binding [MESH]Theoretical limitations of quantification for noncompetitive sandwich (epmc).pdf DeepDyve Pubget Overpricing