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The tetraspanin web revisited by super-resolution microscopy
#MMPMID26183063
Zuidscherwoude M
; Göttfert F
; Dunlock VM
; Figdor CG
; van den Bogaart G
; van Spriel AB
Sci Rep
2015[Jul]; 5
(?): 12201
PMID26183063
show ga
The spatial organization of membrane proteins in the plasma membrane is critical
for signal transduction, cell communication and membrane trafficking.
Tetraspanins organize functional higher-order protein complexes called
'tetraspanin-enriched microdomains (TEMs)' via interactions with partner
molecules and other tetraspanins. Still, the nanoscale organization of TEMs in
native plasma membranes has not been resolved. Here, we elucidated the size,
density and distribution of TEMs in the plasma membrane of human B cells and
dendritic cells using dual color stimulated emission depletion (STED) microscopy.
We demonstrate that tetraspanins form individual nanoclusters smaller than 120?nm
and quantified that a single tetraspanin CD53 cluster contains less than ten CD53
molecules. CD53 and CD37 domains were adjacent to and displayed only minor
overlap with clusters containing tetraspanins CD81 or CD82. Moreover, CD53 and
CD81 were found in closer proximity to their partners MHC class II and CD19 than
to other tetraspanins. Although these results indicate that tetraspanin domains
are adjacently positioned in the plasma membrane, they challenge the current view
of the tetraspanin web of multiple tetraspanin species organized into a single
domain. This study increases the molecular understanding of TEMs at the nanoscale
level which is essential for comprehending tetraspanin function in cell biology.
|*Microscopy, Fluorescence
[MESH]
|Antigens, CD19/metabolism
[MESH]
|B-Lymphocytes/metabolism
[MESH]
|Cell Line
[MESH]
|Cell Membrane/metabolism
[MESH]
|Dendritic Cells/metabolism
[MESH]
|Histocompatibility Antigens Class II/metabolism
[MESH]
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