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10.1126/sciadv.1500734 http://scihub22266oqcxt.onion/10.1126/sciadv.1500734 C4643809!4643809 !26601243     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26601243 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26601243 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Adv 2015 ; 1 (7 ): e1500734 Nephropedia Template TP {{tp|p=26601243 |t=2015. The structure of DNA by direct imaging |pdf=|usr=}}{{26601243 }} gab.com Text The structure of DNA by direct imaging JO: Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=26601243 #FOAvip The structure of DNA was determined in 1953 by x-ray fiber diffraction. Several attempts have been made to obtain a direct image of DNA with alternative techniques. The direct image is intended to allow a quantitative evaluation of all relevant characteristic lengths present in a molecule. A direct image of DNA, which is different from diffraction in the reciprocal space, is difficult to obtain for two main reasons: the intrinsic very low contrast of the elements that form the molecule and the difficulty of preparing the sample while preserving its pristine shape and size. We show that through a preparation procedure compatible with the DNA physiological conditions, a direct image of a single suspended DNA molecule can be obtained. In the image, all relevant lengths of A-form DNA are measurable. A high-resolution transmission electron microscope that operates at 80 keV with an ultimate resolution of 1.5 Å was used for this experiment. Direct imaging of a single molecule can be used as a method to address biological problems that require knowledge at the single-molecule level, given that the average information obtained by x-ray diffraction of crystals or fibers is not sufficient for detailed structure determination, or when crystals cannot be obtained from biological molecules or are not sufficient in understanding multiple protein configurations. Twit Text FOAVip The structure of DNA by direct imaging ????Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=26601243 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26601243 #FOAvip English Wikipedia <ref>{{Cite pmid|26601243 }}</ref> The structure of DNA by direct imaging #MMPMID26601243 Marini M ; Falqui A ; Moretti M ; Limongi T ; Allione M ; Genovese A ; Lopatin S ; Tirinato L ; Das G ; Torre B ; Giugni A ; Gentile F ; Candeloro P ; Di Fabrizio E Sci Adv 2015[Aug]; 1 (7 ): e1500734 PMID26601243 show ga The structure of DNA was determined in 1953 by x-ray fiber diffraction. Several attempts have been made to obtain a direct image of DNA with alternative techniques. The direct image is intended to allow a quantitative evaluation of all relevant characteristic lengths present in a molecule. A direct image of DNA, which is different from diffraction in the reciprocal space, is difficult to obtain for two main reasons: the intrinsic very low contrast of the elements that form the molecule and the difficulty of preparing the sample while preserving its pristine shape and size. We show that through a preparation procedure compatible with the DNA physiological conditions, a direct image of a single suspended DNA molecule can be obtained. In the image, all relevant lengths of A-form DNA are measurable. A high-resolution transmission electron microscope that operates at 80 keV with an ultimate resolution of 1.5 Å was used for this experiment. Direct imaging of a single molecule can be used as a method to address biological problems that require knowledge at the single-molecule level, given that the average information obtained by x-ray diffraction of crystals or fibers is not sufficient for detailed structure determination, or when crystals cannot be obtained from biological molecules or are not sufficient in understanding multiple protein configurations. äThe structure of DNA by direct imaging (epmc).pdf DeepDyve Pubget Overpricing