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2017 ; 104
(5
): 752-760
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The processive kinetics of gene conversion in bacteria
#MMPMID28256783
Paulsson J
; El Karoui M
; Lindell M
; Hughes D
Mol Microbiol
2017[Jun]; 104
(5
): 752-760
PMID28256783
show ga
Gene conversion, non-reciprocal transfer from one homologous sequence to another,
is a major force in evolutionary dynamics, promoting co-evolution in gene
families and maintaining similarities between repeated genes. However, the
properties of the transfer - where it initiates, how far it proceeds and how the
resulting conversion tracts are affected by mismatch repair - are not well
understood. Here, we use the duplicate tuf genes in Salmonella as a
quantitatively tractable model system for gene conversion. We selected for
conversion in multiple different positions of tuf, and examined the resulting
distributions of conversion tracts in mismatch repair-deficient and mismatch
repair-proficient strains. A simple stochastic model accounting for the essential
steps of conversion showed excellent agreement with the data for all selection
points using the same value of the conversion processivity, which is the only
kinetic parameter of the model. The analysis suggests that gene conversion
effectively initiates uniformly at any position within a tuf gene, and proceeds
with an effectively uniform conversion processivity in either direction limited
by the bounds of the gene.
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