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2017 ; 7
(1
): 1652
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The intestinal tuft cell nanostructure in 3D
#MMPMID28490731
Hoover B
; Baena V
; Kaelberer MM
; Getaneh F
; Chinchilla S
; Bohórquez DV
Sci Rep
2017[May]; 7
(1
): 1652
PMID28490731
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Once referred to as "peculiar," tuft cells are enigmatic epithelial cells. Here,
we reasoned that future functional studies could be derived from a complete
account of the tuft cell ultrastructure. We identified and documented the
volumetric ultrastructure at nanometer resolution (4-5?nm/pixel) of specific
intestinal tuft cells. The techniques used were Serial Block-Face (SBF) and
Automated Tape-collecting Ultra-Microtome (ATUM) Scanning Electron Microscopy
(SEM). Our results exposed a short (~15?µm) basal cytoplasmic process devoid of
secretory vesicles. Volume rendering of serial sections unveiled several thin
cytospinules (~1?µm). These cytospinules project from the tuft cell into the
nuclei of neighboring epithelial cells. Volume rendering also revealed within the
tuft cell an elegant network of interconnected tubules. The network forms a
passage from the base of the microvilli to the rough endoplasmic reticulum. Based
on their location and microanatomy, the tuft cells' cytospinules, and tubular
network, might facilitate the exchange of molecular cargo with nuclei of
neighboring cells, and the gut lumen.
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