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2014 ; 28
(11
): 4583-90
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The importance of being a lumen
#MMPMID25077562
Bischel LL
; Sung KE
; Jiménez-Torres JA
; Mader B
; Keely PJ
; Beebe DJ
FASEB J
2014[Nov]; 28
(11
): 4583-90
PMID25077562
show ga
Advances in tissue engineering and microtechnology have enabled researchers to
more easily generate in vitro tissue models that mimic the tissue geometry and
spatial organization found in vivo (e.g., vessel or mammary duct models with
tubular structures). However, the widespread adoption of these models for
biological studies has been slow, in part due to the lack of direct comparisons
between existing 2-dimensional and 3-dimensional cell culture models and new
organotypic models that better replicate tissue structure. Using previously
developed vessel and mammary duct models with 3-dimensional lumen structures, we
have begun to explore this question. In a direct comparison between these next
generation organotypic models and more traditional methods, we observed
differences in the levels of several secreted growth factors and cytokines. In
addition, endothelial vessel geometry profoundly affects the phenotypic behavior
of carcinoma cells, suggesting that more traditional in vitro assays may not
capture in vivo events. Here, we seek to review and add to the increasing
evidence supporting the hypothesis that using cell culture models with more
relevant tissue structure influences cell fate and behavior, potentially
increasing the relevance of biological findings.
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