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2014 ; 20
(ä): 103-11
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The bright future of single-molecule fluorescence imaging
#MMPMID24956235
Juette MF
; Terry DS
; Wasserman MR
; Zhou Z
; Altman RB
; Zheng Q
; Blanchard SC
Curr Opin Chem Biol
2014[Jun]; 20
(ä): 103-11
PMID24956235
show ga
Single-molecule Förster resonance energy transfer (smFRET) is an essential and
maturing tool to probe biomolecular interactions and conformational dynamics in
vitro and, increasingly, in living cells. Multi-color smFRET enables the
correlation of multiple such events and the precise dissection of their order and
timing. However, the requirements for good spectral separation, high time
resolution, and extended observation times place extraordinary demands on the
fluorescent labels used in such experiments. Together with advanced experimental
designs and data analysis, the development of long-lasting, non-fluctuating
fluorophores is therefore proving key to progress in the field. Recently
developed strategies for obtaining ultra-stable organic fluorophores spanning the
visible spectrum are underway that will enable multi-color smFRET studies to
deliver on their promise of previously unachievable biological insights.
|Color
[MESH]
|Fluorescence Resonance Energy Transfer/*methods
[MESH]