Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1074/jbc.M115.684035 http://scihub22266oqcxt.onion/10.1074/jbc.M115.684035 C4646019!4646019 !26424794     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26424794 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Biol+Chem 2015 ; 290 (46 ): 27700-11 Nephropedia Template TP {{tp|p=26424794 |t=2015. The Silent Sway of Splicing by Synonymous Substitutions |pdf=|usr=}}{{26424794 }} gab.com Text The Silent Sway of Splicing by Synonymous Substitutions JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=26424794 #FOAvip Alternative splicing diversifies mRNA transcripts in human cells. This sequence-driven process can be influenced greatly by mutations, even those that do not change the protein coding potential of the transcript. Synonymous mutations have been shown to alter gene expression through modulation of splicing, mRNA stability, and translation. Using a synonymous position mutation library in SMN1 exon 7, we show that 23% of synonymous mutations across the exon decrease exon inclusion, suggesting that nucleotide identity across the entire exon has been evolutionarily optimized to support a particular exon inclusion level. Although phylogenetic conservation scores are insufficient to identify synonymous positions important for exon inclusion, an alignment of organisms filtered based on similar exon/intron architecture is highly successful. Although many of the splicing neutral mutations are observed to occur, none of the exon inclusion reducing mutants was found in the filtered alignment. Using the modified phylogenetic comparison as an approach to evaluate the impact on pre-mRNA splicing suggests that up to 45% of synonymous SNPs are likely to alter pre-mRNA splicing. These results demonstrate that coding and pre-mRNA splicing pressures co-evolve and that a modified phylogenetic comparison based on the exon/intron architecture is a useful tool in identifying splice altering SNPs. Twit Text FOAVip The Silent Sway of Splicing by Synonymous Substitutions ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=26424794 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26424794 #FOAvip English Wikipedia <ref>{{Cite pmid|26424794 }}</ref> The Silent Sway of Splicing by Synonymous Substitutions #MMPMID26424794 Mueller WF ; Larsen LS ; Garibaldi A ; Hatfield GW ; Hertel KJ J Biol Chem 2015[Nov]; 290 (46 ): 27700-11 PMID26424794 show ga Alternative splicing diversifies mRNA transcripts in human cells. This sequence-driven process can be influenced greatly by mutations, even those that do not change the protein coding potential of the transcript. Synonymous mutations have been shown to alter gene expression through modulation of splicing, mRNA stability, and translation. Using a synonymous position mutation library in SMN1 exon 7, we show that 23% of synonymous mutations across the exon decrease exon inclusion, suggesting that nucleotide identity across the entire exon has been evolutionarily optimized to support a particular exon inclusion level. Although phylogenetic conservation scores are insufficient to identify synonymous positions important for exon inclusion, an alignment of organisms filtered based on similar exon/intron architecture is highly successful. Although many of the splicing neutral mutations are observed to occur, none of the exon inclusion reducing mutants was found in the filtered alignment. Using the modified phylogenetic comparison as an approach to evaluate the impact on pre-mRNA splicing suggests that up to 45% of synonymous SNPs are likely to alter pre-mRNA splicing. These results demonstrate that coding and pre-mRNA splicing pressures co-evolve and that a modified phylogenetic comparison based on the exon/intron architecture is a useful tool in identifying splice altering SNPs. |*Mutation [MESH] |Alternative Splicing/*genetics [MESH] |Animals [MESH] |Base Sequence [MESH] |DNA Mutational Analysis [MESH] |Exons [MESH] |Gene Library [MESH] |HeLa Cells [MESH] |Humans [MESH] |Introns [MESH] |Molecular Sequence Data [MESH] |Phylogeny [MESH] |RNA Precursors/genetics [MESH] |RNA, Messenger/*genetics [MESH]The Silent Sway of Splicing by Synonymous Substitutions (epmc).pdf DeepDyve Pubget Overpricing