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10.1016/j.chembiol.2017.06.007

http://scihub22266oqcxt.onion/10.1016/j.chembiol.2017.06.007
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suck abstract from ncbi


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pmid28712745
      Cell+Chem+Biol 2017 ; 24 (7 ): 870-880.e5
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  • The Ligand Binding Landscape of Diacylglycerol Kinases #MMPMID28712745
  • Franks CE ; Campbell ST ; Purow BW ; Harris TE ; Hsu KL
  • Cell Chem Biol 2017[Jul]; 24 (7 ): 870-880.e5 PMID28712745 show ga
  • Diacylglycerol kinases (DGKs) are integral components of signal transduction cascades that regulate cell biology through ATP-dependent phosphorylation of the lipid messenger diacylglycerol. Methods for direct evaluation of DGK activity in native biological systems are lacking and needed to study isoform-specific functions of these multidomain lipid kinases. Here, we utilize ATP acyl phosphate activity-based probes and quantitative mass spectrometry to define, for the first time, ATP and small-molecule binding motifs of representative members from all five DGK subtypes. We use chemical proteomics to discover an unusual binding mode for the DGK? inhibitor, ritanserin, including interactions at the atypical C1 domain distinct from the ATP binding region. Unexpectedly, deconstruction of ritanserin yielded a fragment compound that blocks DGK? activity through a conserved binding mode and enhanced selectivity against the kinome. Collectively, our studies illustrate the power of chemical proteomics to profile protein-small molecule interactions of lipid kinases for fragment-based lead discovery.
  • |*Ligands [MESH]
  • |Adenosine Triphosphate/chemistry/metabolism [MESH]
  • |Binding Sites [MESH]
  • |Chromatography, High Pressure Liquid [MESH]
  • |Diacylglycerol Kinase/chemistry/genetics/*metabolism [MESH]
  • |HEK293 Cells [MESH]
  • |Humans [MESH]
  • |Isotope Labeling [MESH]
  • |Ketanserin/chemistry/metabolism [MESH]
  • |Peptides/analysis [MESH]
  • |Protein Binding [MESH]
  • |Protein Isoforms/chemistry/genetics/metabolism [MESH]
  • |Proteome/analysis [MESH]
  • |Recombinant Proteins/biosynthesis/chemistry/isolation & purification [MESH]
  • |Ritanserin/chemistry/metabolism [MESH]


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