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10.3390/ijms17040471 http://scihub22266oqcxt.onion/10.3390/ijms17040471 C4848927!4848927 !27043537     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27043537 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Int+J+Mol+Sci 2016 ; 17 (4 ): 471 Nephropedia Template TP {{tp|p=27043537 |t=2016. The Expression Profile of Complement Components in Podocytes |pdf=|usr=}}{{27043537 }} gab.com Text The Expression Profile of Complement Components in Podocytes JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=27043537 #FOAvip Podocytes are critical for maintaining the glomerular filtration barrier and are injured in many renal diseases, especially proteinuric kidney diseases. Recently, reports suggested that podocytes are among the renal cells that synthesize complement components that mediate glomerular diseases. Nevertheless, the profile and extent of complement component expression in podocytes remain unclear. This study examined the expression profile of complement in podocytes under physiological conditions and in abnormal podocytes induced by multiple stimuli. In total, 23/32 complement component components were detected in podocyte by conventional RT-PCR. Both primary cultured podocytes and immortalized podocytes expressed the complement factors C1q, C1r, C2, C3, C7, MASP, CFI, DAF, CD59, C4bp, CD46, Protein S, CR2, C1qR, C3aR, C5aR, and Crry (17/32), whereas C4, CFB, CFD, C5, C6, C8, C9, MBL1, and MBL2 (9/32) complement factors were not expressed. C3, Crry, and C1q-binding protein were detected by tandem mass spectrometry. Podocyte complement gene expression was affected by several factors (puromycin aminonucleoside (PAN), angiotensin II (Ang II), interleukin-6 (IL-6), and transforming growth factor-? (TGF-?)). Representative complement components were detected using fluorescence confocal microscopy. In conclusion, primary podocytes express various complement components at the mRNA and protein levels. The complement gene expressions were affected by several podocyte injury factors. Twit Text FOAVip The Expression Profile of Complement Components in Podocytes ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=27043537 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27043537 #FOAvip English Wikipedia <ref>{{Cite pmid|27043537 }}</ref> The Expression Profile of Complement Components in Podocytes #MMPMID27043537 Li X ; Ding F ; Zhang X ; Li B ; Ding J Int J Mol Sci 2016[Mar]; 17 (4 ): 471 PMID27043537 show ga Podocytes are critical for maintaining the glomerular filtration barrier and are injured in many renal diseases, especially proteinuric kidney diseases. Recently, reports suggested that podocytes are among the renal cells that synthesize complement components that mediate glomerular diseases. Nevertheless, the profile and extent of complement component expression in podocytes remain unclear. This study examined the expression profile of complement in podocytes under physiological conditions and in abnormal podocytes induced by multiple stimuli. In total, 23/32 complement component components were detected in podocyte by conventional RT-PCR. Both primary cultured podocytes and immortalized podocytes expressed the complement factors C1q, C1r, C2, C3, C7, MASP, CFI, DAF, CD59, C4bp, CD46, Protein S, CR2, C1qR, C3aR, C5aR, and Crry (17/32), whereas C4, CFB, CFD, C5, C6, C8, C9, MBL1, and MBL2 (9/32) complement factors were not expressed. C3, Crry, and C1q-binding protein were detected by tandem mass spectrometry. Podocyte complement gene expression was affected by several factors (puromycin aminonucleoside (PAN), angiotensin II (Ang II), interleukin-6 (IL-6), and transforming growth factor-? (TGF-?)). Representative complement components were detected using fluorescence confocal microscopy. In conclusion, primary podocytes express various complement components at the mRNA and protein levels. The complement gene expressions were affected by several podocyte injury factors. |*Transcriptome/drug effects [MESH] |Angiotensin II/genetics/metabolism [MESH] |Animals [MESH] |Carrier Proteins/genetics/metabolism [MESH] |Cells, Cultured [MESH] |Complement System Proteins/analysis/genetics/*metabolism [MESH] |Diabetic Nephropathies/metabolism/pathology [MESH] |Disease Models, Animal [MESH] |Humans [MESH] |Interleukin-6/genetics/metabolism [MESH] |Male [MESH] |Microfilament Proteins/genetics/metabolism [MESH] |Microscopy, Confocal [MESH] |Podocytes/cytology/metabolism [MESH] |Puromycin Aminonucleoside/pharmacology [MESH] |Rats [MESH] |Rats, Sprague-Dawley [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Tandem Mass Spectrometry [MESH]The Expression Profile of Complement Components in Podocytes (epmc).pdf DeepDyve Pubget Overpricing