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10.1073/pnas.1610177113 http://scihub22266oqcxt.onion/10.1073/pnas.1610177113 C5081628!5081628 !27708162     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27708162 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2016 ; 113 (42 ): E6476-E6485 Nephropedia Template TP {{tp|p=27708162 |t=2016. The Architecture of Trypanosoma brucei editosomes |pdf=|usr=}}{{27708162 }} gab.com Text The Architecture of Trypanosoma brucei editosomes JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=27708162 #FOAvip Uridine insertion and deletion RNA editing generates functional mitochondrial mRNAs in Trypanosoma brucei Editing is catalyzed by three distinct ?20S editosomes that have a common set of 12 proteins, but are typified by mutually exclusive RNase III endonucleases with distinct cleavage specificities and unique partner proteins. Previous studies identified a network of protein-protein interactions among a subset of common editosome proteins, but interactions among the endonucleases and their partner proteins, and their interactions with common subunits were not identified. Here, chemical cross-linking and mass spectrometry, comparative structural modeling, and genetic and biochemical analyses were used to define the molecular architecture and subunit organization of purified editosomes. We identified intra- and interprotein cross-links for all editosome subunits that are fully consistent with editosome protein structures and previously identified interactions, which we validated by genetic and biochemical studies. The results were used to create a highly detailed map of editosome protein domain proximities, leading to identification of molecular interactions between subunits, insights into the functions of noncatalytic editosome proteins, and a global understanding of editosome architecture. Twit Text FOAVip The Architecture of Trypanosoma brucei editosomes ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=27708162 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27708162 #FOAvip English Wikipedia <ref>{{Cite pmid|27708162 }}</ref> The Architecture of Trypanosoma brucei editosomes #MMPMID27708162 McDermott SM ; Luo J ; Carnes J ; Ranish JA ; Stuart K Proc Natl Acad Sci U S A 2016[Oct]; 113 (42 ): E6476-E6485 PMID27708162 show ga Uridine insertion and deletion RNA editing generates functional mitochondrial mRNAs in Trypanosoma brucei Editing is catalyzed by three distinct ?20S editosomes that have a common set of 12 proteins, but are typified by mutually exclusive RNase III endonucleases with distinct cleavage specificities and unique partner proteins. Previous studies identified a network of protein-protein interactions among a subset of common editosome proteins, but interactions among the endonucleases and their partner proteins, and their interactions with common subunits were not identified. Here, chemical cross-linking and mass spectrometry, comparative structural modeling, and genetic and biochemical analyses were used to define the molecular architecture and subunit organization of purified editosomes. We identified intra- and interprotein cross-links for all editosome subunits that are fully consistent with editosome protein structures and previously identified interactions, which we validated by genetic and biochemical studies. The results were used to create a highly detailed map of editosome protein domain proximities, leading to identification of molecular interactions between subunits, insights into the functions of noncatalytic editosome proteins, and a global understanding of editosome architecture. |*RNA Editing [MESH] |Computational Biology/methods [MESH] |Endonucleases/metabolism [MESH] |Models, Molecular [MESH] |Multiprotein Complexes/metabolism [MESH] |Protein Binding [MESH] |Protein Conformation [MESH] |Protein Interaction Domains and Motifs [MESH] |Protein Interaction Maps [MESH] |Proteome [MESH] |Proteomics/methods [MESH] |Protozoan Proteins/chemistry/*metabolism [MESH] |RNA, Protozoan/*genetics [MESH] |RNA-Binding Proteins/metabolism [MESH] |Reproducibility of Results [MESH]The Architecture of Trypanosoma brucei editosomes (epmc).pdf DeepDyve Pubget Overpricing