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2020 ; 209
(4
): 489-498
Nephropedia Template TP
Benayas B
; Sastre I
; López-Martín S
; Oo A
; Kim B
; Bullido MJ
; Aldudo J
; Yáńez-Mó M
Med Microbiol Immunol
2020[Aug]; 209
(4
): 489-498
PMID32500359
show ga
Different members of the tetraspanin superfamily have been described to regulate
different virus infectious cycles at several stages: viral entry, viral
replication or virion exit or infectivity. In addition, tetraspanin CD81
regulates HIV reverse transcription through its association with the dNTP
hydrolase SAMHD1. Here we aimed at analysing the role of CD81 in Herpes simplex
virus 1 infectivity using a neuroblastoma cell model. For this purpose, we
generated a CD81 KO cell line using the CRISPR/Cas9 technology. Despite being
CD81 a plasma membrane protein, CD81 KO cells showed no defects in viral entry
nor in the expression of early protein markers. In contrast, glycoprotein B and
C, which require viral DNA replication for their expression, were significantly
reduced in CD81 KO infected cells. Indeed, HSV-1 DNA replication and the
formation of new infectious particles were severely compromised in CD81 KO cells.
We could not detect significant changes in SAMHD1 total expression levels, but a
relocalization into endosomal structures was observed in CD81 KO cells. In
summary, CD81 KO cells showed impaired viral DNA replication and produced greatly
diminished viral titers.
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