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2010 ; 14
(8
): 2085-93
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Telocytes in human epicardium
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Popescu LM
; Manole CG
; Gherghiceanu M
; Ardelean A
; Nicolescu MI
; Hinescu ME
; Kostin S
J Cell Mol Med
2010[Aug]; 14
(8
): 2085-93
PMID20629996
show ga
The existence of the epicardial telocytes was previously documented by
immunohistochemistry (IHC) or immunofluorescence. We have also demonstrated
recently that telocytes are present in mice epicardium, within the cardiac
stem-cell niches, and, possibly, they are acting as nurse cells for the
cardiomyocyte progenitors. The rationale of this study was to show that telocytes
do exist in human (sub)epicardium, too. Human autopsy hearts from 10 adults and
15 foetuses were used for conventional IHC for c-kit/CD117, CD34, vimentin,
S-100, ?, Neurokinin 1, as well as using laser confocal microscopy. Tissue
samples obtained by surgical biopsies from 10 adults were studied by digital
transmission electron microscopy (TEM). Double immunolabelling for c-kit/CD34
and, for c-kit/vimentin suggests that in human beings, epicardial telocytes share
similar immunophenotype features with myocardial telocytes. The presence of the
telocytes in human epicardium is shown by TEM. Epicardial telocytes, like any of
the telocytes are defined by telopodes, their cell prolongations, which are very
long (several tens of ?m), very thin (0.1-0.2 ?m, below the resolving power of
light microscopy) and with moniliform configuration. The interconnected
epicardial telocytes create a 3D cellular network, connected with the 3D network
of myocardial telocytes. TEM documented that telocytes release shed microvesicles
or exocytotic multivesicular bodies in the intercellular space. The human
epicardial telocytes have similar phenotype (TEM and IHC) with telocytes located
among human working cardiomyocyte. It remains to be established the role(s) of
telocytes in cardiac renewing/repair/regeneration processes, and also the
pathological aspects induced by their 'functional inhibition', or by their
variation in number. We consider telocytes as a real candidate for future
developments of autologous cell-based therapy in heart diseases.
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