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2014 ; 20
(6
): 669-82
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Technology advancement for integrative stem cell analyses
#MMPMID24874188
Jeong Y
; Choi J
; Lee KH
Tissue Eng Part B Rev
2014[Dec]; 20
(6
): 669-82
PMID24874188
show ga
Scientists have endeavored to use stem cells for a variety of applications
ranging from basic science research to translational medicine. Population-based
characterization of such stem cells, while providing an important foundation to
further development, often disregard the heterogeneity inherent among individual
constituents within a given population. The population-based analysis and
characterization of stem cells and the problems associated with such a blanket
approach only underscore the need for the development of new analytical
technology. In this article, we review current stem cell analytical technologies,
along with the advantages and disadvantages of each, followed by applications of
these technologies in the field of stem cells. Furthermore, while recent advances
in micro/nano technology have led to a growth in the stem cell analytical field,
underlying architectural concepts allow only for a vertical analytical approach,
in which different desirable parameters are obtained from multiple individual
experiments and there are many technical challenges that limit vertically
integrated analytical tools. Therefore, we propose--by introducing a concept of
vertical and horizontal approach--that there is the need of adequate methods to
the integration of information, such that multiple descriptive parameters from a
stem cell can be obtained from a single experiment.