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10.1007/978-1-4939-0992-6_8

http://scihub22266oqcxt.onion/10.1007/978-1-4939-0992-6_8
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C5111905!5111905 !25030921
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suck abstract from ncbi


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pmid25030921
      Methods+Mol+Biol 2014 ; 1176 (ä): 89-106
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  • Targeted genome modification via triple helix formation #MMPMID25030921
  • Ricciardi AS ; McNeer NA ; Anandalingam KK ; Saltzman WM ; Glazer PM
  • Methods Mol Biol 2014[]; 1176 (ä): 89-106 PMID25030921 show ga
  • Triplex-forming oligonucleotides (TFOs) are capable of coordinating genome modification in a targeted, site-specific manner, causing mutagenesis or even coordinating homologous recombination events. Here, we describe the use of TFOs such as peptide nucleic acids for targeted genome modification. We discuss this method and its applications and describe protocols for TFO design, delivery, and evaluation of activity in vitro and in vivo.
  • |*Nucleic Acid Conformation [MESH]
  • |Animals [MESH]
  • |Gene Expression [MESH]
  • |Gene Targeting/*methods [MESH]
  • |Genes, Reporter [MESH]
  • |Genome/*genetics [MESH]
  • |High-Throughput Nucleotide Sequencing [MESH]
  • |Humans [MESH]
  • |Mice [MESH]
  • |Mutagenesis [MESH]
  • |Oligonucleotides/chemistry/genetics [MESH]
  • |Peptide Nucleic Acids [MESH]


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