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2015 ; 2
(Pt 2
): 283-91
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Synchrotron radiation macromolecular crystallography: science and spin-offs
#MMPMID25866664
Helliwell JR
; Mitchell EP
IUCrJ
2015[Mar]; 2
(Pt 2
): 283-91
PMID25866664
show ga
A current overview of synchrotron radiation (SR) in macromolecular
crystallography (MX) instrumentation, methods and applications is presented.
Automation has been and remains a central development in the last decade, as have
the rise of remote access and of industrial service provision. Results include a
high number of Protein Data Bank depositions, with an increasing emphasis on the
successful use of microcrystals. One future emphasis involves pushing the
frontiers of using higher and lower photon energies. With the advent of X-ray
free-electron lasers, closely linked to SR developments, the use of ever smaller
samples such as nanocrystals, nanoclusters and single molecules is anticipated,
as well as the opening up of femtosecond time-resolved diffraction structural
studies. At SR sources, a very high-throughput assessment for the best crystal
samples and the ability to tackle just a few micron and sub-micron crystals will
become widespread. With higher speeds and larger detectors, diffraction data
volumes are becoming long-term storage and archiving issues; the implications for
today and the future are discussed. Together with the rise of the storage ring to
its current pre-eminence in MX data provision, the growing tendency of central
facility sites to offer other centralized facilities complementary to
crystallography, such as cryo-electron microscopy and NMR, is a welcome
development.