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10.1038/s41598-018-28178-3 http://scihub22266oqcxt.onion/10.1038/s41598-018-28178-3 C6030211!6030211 !29968790     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=29968790 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29968790 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2018 ; 8 (1 ): 10051 Nephropedia Template TP {{tp|p=29968790 |t=2018. Switchable genome editing via genetic code expansion |pdf=|usr=}}{{29968790 }} gab.com Text Switchable genome editing via genetic code expansion JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29968790 #FOAvip Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light. However, these approaches are often impracticable, for example in clinical therapeutic genome editing in situ or gene drives in which environmentally-compatible control is paramount. With this in mind, we have developed heritable Cas9-mediated mammalian genome editing that is acutely controlled by the cheap lysine derivative, Lys(Boc) (BOC). Genetic code expansion permitted non-physiological BOC incorporation such that Cas9 (Cas9(BOC)) was expressed in a full-length, active form in cultured somatic cells only after BOC exposure. Stringently BOC-dependent, heritable editing of transgenic and native genomic loci occurred when Cas9(BOC) was expressed at the onset of mouse embryonic development from cRNA or Cas9(BOC) transgenic females. The tightly controlled Cas9 editing system reported here promises to have broad applications and is a first step towards purposed, spatiotemporal gene drive regulation over large geographical ranges. Twit Text FOAVip Switchable genome editing via genetic code expansion ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=29968790 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29968790 #FOAvip English Wikipedia <ref>{{Cite pmid|29968790 }}</ref> Switchable genome editing via genetic code expansion #MMPMID29968790 Suzuki T ; Asami M ; Patel SG ; Luk LYP ; Tsai YH ; Perry ACF Sci Rep 2018[Jul]; 8 (1 ): 10051 PMID29968790 show ga Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light. However, these approaches are often impracticable, for example in clinical therapeutic genome editing in situ or gene drives in which environmentally-compatible control is paramount. With this in mind, we have developed heritable Cas9-mediated mammalian genome editing that is acutely controlled by the cheap lysine derivative, Lys(Boc) (BOC). Genetic code expansion permitted non-physiological BOC incorporation such that Cas9 (Cas9(BOC)) was expressed in a full-length, active form in cultured somatic cells only after BOC exposure. Stringently BOC-dependent, heritable editing of transgenic and native genomic loci occurred when Cas9(BOC) was expressed at the onset of mouse embryonic development from cRNA or Cas9(BOC) transgenic females. The tightly controlled Cas9 editing system reported here promises to have broad applications and is a first step towards purposed, spatiotemporal gene drive regulation over large geographical ranges. |Animals [MESH] |CRISPR-Associated Protein 9/*metabolism [MESH] |CRISPR-Cas Systems/*genetics [MESH] |Clustered Regularly Interspaced Short Palindromic Repeats [MESH] |Female [MESH] |Gene Editing/*methods [MESH] |Gene Expression Regulation/genetics [MESH] |Genetic Code/genetics [MESH] |Genetic Vectors/genetics [MESH] |Lysine/analogs & derivatives [MESH] |Male [MESH] |Mice [MESH] |Mice, Transgenic/embryology [MESH]Switchable genome editing via genetic code expansion (epmc).pdf DeepDyve Pubget Overpricing